{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:51:57Z","timestamp":1765806717271,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2008,9,19]],"date-time":"2008-09-19T00:00:00Z","timestamp":1221782400000},"content-version":"vor","delay-in-days":49,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple and rapid micellar electrokinetic capillary chromatographic method is described for the separation and quantification of five expectorant drugs, including ambroxol (AMB), bromhexine (BRM), carbocysteine, guaiacol and guaifenesin. The drugs were separated in a mixed solution of phosphate buffer (35\u2009mM; pH 3.90) and acetonitrile (75:25, v\/v) with sodium dodecylsulfate (120\u2009mM) as the micellar source, and the separated drugs were directly monitored with UV detector (200\u2009nm). Several key parameters affecting the separation and analysis of the drugs were studied and optimized. Based on the corrected peak\u2010area ratios of the drugs to an internal standard (7\u2010hydroxy\u20104\u2010methyl\u2010coumarin) <jats:italic>versus<\/jats:italic> the concentration of the drugs, the established method is applicable to quantify AMB and BRM each over 20\u2013150\u2009\u00b5M, carbocysteine over 100\u20131500\u2009\u00b5M, guaiacol and guaifenesin each over 10\u2013150\u2009\u00b5M. The detection limits (<jats:italic>S<\/jats:italic>\/<jats:italic>N<\/jats:italic>=3; 0.5\u2009psi, 5\u2009s injection) of the method for the analytes are in the range of 3.0\u20135.0\u2009\u00b5M (except that of carbocysteine at 30\u2009\u00b5M). The precision (relative standard deviation) and accuracy (relative error) of the method for the intra\u2010day (<jats:italic>n<\/jats:italic>=3) and inter\u2010day (<jats:italic>n<\/jats:italic>=5) analyses of the analytes at three levels are all below 4%. The method is speedy with a run time of about 6\u2009min for the analysis of the five analytes. Application of this method to the analysis of AMB and BRM in pharmaceutical preparations or AMB in the urine of a dosed subject proved simple and effective.<\/jats:p>","DOI":"10.1002\/elps.200800129","type":"journal-article","created":{"date-parts":[[2008,9,19]],"date-time":"2008-09-19T02:37:45Z","timestamp":1221791865000},"page":"3524-3530","source":"Crossref","is-referenced-by-count":7,"title":["A simple micellar electrokinetic capillary chromatographic method for the quantitative analysis of organic expectorants"],"prefix":"10.1002","volume":"29","author":[{"given":"Yu\u2010Ting","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwang\u2010Shang","family":"Kou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Lung","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,19]]},"reference":[{"volume-title":"Pharmacology: A 2\u2010in\u20101 Reference for Nurses","year":"2004","key":"e_1_2_6_2_2"},{"volume-title":"The Merck Index","year":"2006","key":"e_1_2_6_3_2"},{"volume-title":"British Pharmacopoeia","key":"e_1_2_6_4_2"},{"volume-title":"European Pharmacopoeia","year":"2001","key":"e_1_2_6_5_2"},{"volume-title":"Chinese Pharmacopeia","key":"e_1_2_6_6_2"},{"volume-title":"U.S. Pharmacepeia 28\u2010National Formulary 23","year":"2005","key":"e_1_2_6_7_2"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00482-3"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)00722-1"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2004.11.011"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00150-X"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410315"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80025-7"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)82313-0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)80461-2"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80400-0"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2006.07.011"},{"key":"e_1_2_6_18_2","first-page":"35","volume-title":"Pharmaceutical Analysis","author":"Watson D. 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