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The influence of composition of polymerization mixture was optimized in terms of porogenic solvent, monomers\/porogens and monomer\/crosslinker ratios. The composition of the mobile phase was also studied. The optimum monolith was obtained with lauryl methacrylate monomer at 60:40% (wt:wt) lauryl methacrylate\/ethylene dimethacrylate ratio and 60\u2009wt% porogens with 20\u2009wt% of 1,4\u2010butanediol (12\u2009wt% 1,4\u2010butanediol in the polymerization mixture). Excellent resolution between sterols was achieved in less than 7\u2009min with an 85:10:5\u2009v\/v\/v ACN\u20132\u2010propanol\u2013water buffer containing 5\u2009mM Tris at pH 8.0. The limits of detection were lower than 0.04\u2009mM, and inter\u2010day and column\u2010to\u2010column reproducibilities at 0.75\u2009mM were better than 6.2%. The method was applied to the determination of sterols in vegetable oils with different botanical origins and to detect olive oil adulteration with sunflower and soybean oils.<\/jats:p>","DOI":"10.1002\/elps.200800247","type":"journal-article","created":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T01:56:34Z","timestamp":1227664594000},"page":"4603-4611","source":"Crossref","is-referenced-by-count":29,"title":["Rapid determination of sterols in vegetable oils by CEC using methacrylate ester\u2010based monolithic columns"],"prefix":"10.1002","volume":"29","author":[{"given":"Mar\u00eda Jes\u00fas","family":"Lerma\u2010Garc\u00eda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernesto F.","family":"Sim\u00f3\u2010Alfonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Ramis\u2010Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Herrero\u2010Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,11,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2006.01.062"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2005.06.033"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2005.11.007"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1081\/FRI-100104702"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00355-1"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.1116"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2670(94)00464-W"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)01397-3"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-005-0249-x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.17221\/6584-CJFS","volume":"19","author":"Bohacenko I.","year":"2001","journal-title":"Czech J. 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