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For generation of EOF in CEC, different amounts of water were added to <jats:italic>n<\/jats:italic>\u2010hexane\u2013isopropanol (IPA) eluents. Influences of added water and composition of polar modifier on retention, enantioselectivity and resolution were studied. Generally better separation was obtained in the eluents of lower water content. Shorter retention and better enantioselectivities were observed in the eluents of higher IPA content, whereas resolution became lower as IPA content was increased. 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