{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:47:30Z","timestamp":1780537650228,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2008,11,17]],"date-time":"2008-11-17T00:00:00Z","timestamp":1226880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The superoxide dismutases (SOD) are a family of enzymes that function as the first line of antioxidant defense against highly reactive superoxide radicals. In <jats:italic>SOD<\/jats:italic> genes, a number of SNP have been identified and their associations with various diseases have been reported. In the present study, we applied a multiplex single base extension technique to genotype multiple non\u2010synonymous SNP in the <jats:italic>SOD1<\/jats:italic>, <jats:italic>SOD2<\/jats:italic> and <jats:italic>SOD3<\/jats:italic> genes simultaneously, and examined allele distributions in healthy Caucasian (German), Asian (Japanese) and African (Xhosa) populations. Of the ten SNP investigated, two (<jats:italic>SOD2<\/jats:italic> Ala16Val, <jats:italic>SOD3<\/jats:italic> Ala58Thr) were polymorphic in all three ethnic groups and the genotype distributions showed significant inter\u2010group differences. On the other hand, a small number of heterozygotes were observed for three SNP (<jats:italic>SOD2<\/jats:italic> Ser10Ile, <jats:italic>SOD3<\/jats:italic> Ala91Thr, <jats:italic>SOD3<\/jats:italic> Arg231Gly) and no heterogeneity was observed for the remaining five (<jats:italic>SOD1<\/jats:italic> Thr40Ile, <jats:italic>SOD1<\/jats:italic> Asn87Ser, <jats:italic>SOD2<\/jats:italic> Arg156Trp, <jats:italic>SOD2<\/jats:italic> Gly76Arg, <jats:italic>SOD2<\/jats:italic> Glu66Val). Analyses of associations between <jats:italic>SOD<\/jats:italic> genotypes and levels of plasma SOD activity demonstrated that <jats:italic>SOD2<\/jats:italic> Ala16Val, a dimorphism leading to substitution in the mitochondrial targeting sequence of SOD2, significantly influences plasma SOD2 activity, and that <jats:italic>SOD3<\/jats:italic> Arg231Gly, leading to substitution in the heparin\u2010binding domain of SOD3, significantly influences plasma total SOD activity.<\/jats:p>","DOI":"10.1002\/elps.200800332","type":"journal-article","created":{"date-parts":[[2008,11,17]],"date-time":"2008-11-17T08:51:21Z","timestamp":1226911881000},"page":"4788-4794","source":"Crossref","is-referenced-by-count":14,"title":["Multiplex single base extension method for simultaneous genotyping of non\u2010synonymous SNP in the three human SOD genes"],"prefix":"10.1002","volume":"29","author":[{"given":"Reiko","family":"Iida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etsuko","family":"Tsubota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruo","family":"Takeshita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,3]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Free Radicals in Biology and Medicine","author":"Halliwell B.","year":"2007"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10405"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39880-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90836-A"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43735-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)92406-N"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210886200"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.08.027"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2940853"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.10.847"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00905-X"},{"key":"e_1_2_6_13_2","first-page":"602","volume":"59","author":"Ambrosone C. B.","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.23.1818"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2004.03.027"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2004.06.027"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1158\/1055-9965.EPI-07-0160","volume":"16","author":"Kang D.","year":"2007","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9998"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2005.05.006"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2003.08.017"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90558-X"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2007.09.014"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1999)13:1<1::AID-HUMU1>3.0.CO;2-I"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/74215"},{"key":"e_1_2_6_25_2","first-page":"19","volume":"492","author":"Marklund S.","year":"1980","journal-title":"Acta Physiol. Scand. Suppl."},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11853"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.9.4471"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-200303000-00004"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/01213011-200505000-00006"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1394"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32289-5"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/3.12.2251"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/22.5.827"},{"key":"e_1_2_6_34_2","first-page":"1222","volume":"91","author":"Lightfoot T. J.","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.2003.005611"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1036"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1159\/000022873"},{"key":"e_1_2_6_38_2","first-page":"321","volume":"54","author":"Salzman R.","year":"2007","journal-title":"Neoplasma"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000105720.28086.6C"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5219"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200800332","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200800332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:16:34Z","timestamp":1760526994000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200800332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":39,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1002\/elps.200800332"],"URL":"https:\/\/doi.org\/10.1002\/elps.200800332","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}