{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T19:03:51Z","timestamp":1769281431775,"version":"3.49.0"},"reference-count":86,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2008,11,7]],"date-time":"2008-11-07T00:00:00Z","timestamp":1226016000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study investigated sequence variation in part of the 60 kilodalton glycoprotein (p<jats:italic>gp60<\/jats:italic>) gene among <jats:italic>Cryptosporidium hominis<\/jats:italic> and <jats:italic>Cryptosporidium parvum<\/jats:italic> isolates (<jats:italic>n<\/jats:italic>=115) from citizens of the UK inferred to have been infected whilst travelling abroad (to 25 countries) or in the UK. The genomic DNA samples from these isolates were subjected to PCR\u2010coupled single\u2010strand conformation polymorphism analysis, followed by targeted sequencing of p<jats:italic>gp60<\/jats:italic>. Individual samples were classified to the genotypic and subgenotypic levels based on phylogenetic analysis (Bayesian inference) of p<jats:italic>gp60<\/jats:italic> data, including published sequences for comparison. Based on this analysis, five <jats:italic>C. hominis<\/jats:italic> (Ia\u2013If) and four <jats:italic>C. parvum<\/jats:italic> (IIa, IIc\u2013IIe) genotypes were identified, equating to 16 and 10 subgenotypes, respectively. Of these genotypes, <jats:italic>C. hominis<\/jats:italic> Ib was predominant (<jats:italic>n<\/jats:italic>=82). Interestingly, one subgenotype (<jats:italic>C. hominis<\/jats:italic> Ib A10G2R2) accounted for the majority of the samples examined and was identified in travellers to 14 countries; the examination of published records suggested that <jats:italic>C. hominis<\/jats:italic> Ib A10G2R2 has a global distribution. Numerous new and seemingly rare subgenotypes (eight for <jats:italic>C. hominis<\/jats:italic> and six for <jats:italic>C. parvum<\/jats:italic>) were also discovered. In conclusion, the present study revealed substantial genetic variation in p<jats:italic>gp60<\/jats:italic> within both <jats:italic>C. hominis<\/jats:italic> and <jats:italic>C. parvum<\/jats:italic> and emphasizes the need to undertake investigations of human and animal populations in countries for which there is no information on the genetic make\u2010up of <jats:italic>Cryptosporidium<\/jats:italic> infecting humans.<\/jats:p>","DOI":"10.1002\/elps.200800422","type":"journal-article","created":{"date-parts":[[2008,11,7]],"date-time":"2008-11-07T04:30:27Z","timestamp":1226032227000},"page":"4119-4129","source":"Crossref","is-referenced-by-count":31,"title":["Analysis of the genetic diversity within <i>Cryptosporidium hominis<\/i> and <i>Cryptosporidium parvum<\/i> from imported and autochtonous cases of human cryptosporidiosis by mutation scanning"],"prefix":"10.1002","volume":"29","author":[{"given":"Aaron R.","family":"Jex","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,11,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182098002765"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00164-8"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.09.004"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.72-97.2004"},{"key":"e_1_2_5_6_2","first-page":"185","volume":"47","author":"Cacci\u00f2 S. 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