{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:50:00Z","timestamp":1790394600197,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,3,23]],"date-time":"2009-03-23T00:00:00Z","timestamp":1237766400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although gene expression following bortezomib treatment has been previously explored, direct effects of bortezomib\u2010induced proteasome inhibition on protein level has not been analyzed so far. Using 2\u2010D PAGE in five mantle cell lymphoma cell lines, we screened for cellular protein level alterations following treatment with 25\u2009nM bortezomib for up to 4\u2009h. Using MS, we identified 38 of the 41 most prominent reliably detected protein spots. Twenty\u2010one were affected in all cell lines, whereas the remaining 20 protein spots were exclusively altered in sensitive cell lines. Western blot analysis was performed for 17 of the 38 identified proteins and 70.6% of the observed protein level alterations in 2\u2010D gels was verified. All cell lines exhibited alterations of the cellular protein levels of heat shock\u2010induced protein species (<jats:italic>HSPA9<\/jats:italic>, <jats:italic>HSP7C<\/jats:italic>, <jats:italic>HSPA5<\/jats:italic>, <jats:italic>HSPD1<\/jats:italic>), whereas sensitive cell lines also displayed altered cellular protein levels of energy metabolism (<jats:italic>ATP5B, AK5, TPI1, ENO\u20101<\/jats:italic>, <jats:italic>ALDOC, GAPDH<\/jats:italic>), RNA and transcriptional regulation <jats:italic>(HNRPL, SFRS12<\/jats:italic>) and cell division (<jats:italic>NEBL, ACTB, SMC1A, C20orf23<\/jats:italic>) as well as tumor suppressor genes (<jats:italic>ENO\u20101<\/jats:italic>, <jats:italic>FH<\/jats:italic>). These proteins clustered in a tight interaction network centered on the major cellular checkpoints TP53. The results were confirmed in primary mantle cell lymphoma, thus confirming the critical role of these candidate proteins of proteasome inhibition.<\/jats:p>","DOI":"10.1002\/elps.200800508","type":"journal-article","created":{"date-parts":[[2009,3,23]],"date-time":"2009-03-23T09:42:42Z","timestamp":1237801362000},"page":"974-986","source":"Crossref","is-referenced-by-count":18,"title":["2\u2010D PAGE\u2010based comparison of proteasome inhibitor bortezomib in sensitive and resistant mantle cell lymphoma"],"prefix":"10.1002","volume":"30","author":[{"given":"Marc","family":"Weinkauf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Zimmermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Rosenwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malte","family":"Rieken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Pastore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grit","family":"Hutter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Hiddemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Dreyling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/10428190500144490"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-05-2091"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdl316"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.07.9665"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.02.050"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.108"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404511"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404959"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.3816\/CLM.2007.n.001"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-12-130781"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-07-034173"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2007.00995.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-007-0543-3"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06132.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(02)00026-7"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501225"},{"key":"e_1_2_6_18_2","first-page":"11","volume":"91","author":"Salaverria I.","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600207"},{"key":"e_1_2_6_20_2","first-page":"2409","volume":"106","author":"Pastore A.","year":"2005","journal-title":"ASH Annual Meeting Abstracts"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg563"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10225"},{"key":"e_1_2_6_24_2","first-page":"1743","volume":"17","author":"Rosenwald A.","year":"2003","journal-title":"Oncology (Huntingt)"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2005.11.013"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.2174\/156720506778249461"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20793"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.04.005"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-03-0494"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-5-161"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01494-0"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1737"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209594"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-4-15"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-050294"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200800508","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200800508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:15:33Z","timestamp":1760523333000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200800508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1002\/elps.200800508"],"URL":"https:\/\/doi.org\/10.1002\/elps.200800508","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3]]}}}