{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:36:16Z","timestamp":1791070576840,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2009,11,2]],"date-time":"2009-11-02T00:00:00Z","timestamp":1257120000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new simple and fast noncovalent coating method based on poly(1\u2010vinylpyrrolidone\u2010co\u20102\u2010dimethylaminoethyl methacrylate) copolymer was developed for CE. Merely 2\u2009min flushing of the capillary with the poly(1\u2010vinylpyrrolidone\u2010co\u20102\u2010dimethylaminoethyl methacrylate) copolymer was required. The copolymer is adsorbed onto the fused\u2010silica surface by hydrogen bonding and electrostatic interactions. EOF was almost totally suppressed over a wide pH range. The coating conditions (flushing time, copolymer concentration, and the concentration and pH of background electrolyte solution) and the stability of the coating were optimized, and the coated capillary was successfully applied to the fast separation of four basic proteins: lysozyme, cytochrome c, ribonuclease A, and \u03b1\u2010chymotrypsinogen A. Separation efficiencies were high, ranging from 386\u2009000 to 738\u2009000\u2009plates\/m at 40\u2009mM pH 4.0 acetate buffer being comparable to values obtained on classical covalent PVP\u2010coated capillary. The RSD of migration times of basic proteins for 200 times successive runs were all below 1.0% (<jats:italic>n<\/jats:italic>=200, 3 days). A successful capillary performance was demonstrated also to the separation of low\u2010 and high\u2010density lipoproteins at acidic pH.<\/jats:p>","DOI":"10.1002\/elps.200900395","type":"journal-article","created":{"date-parts":[[2009,11,2]],"date-time":"2009-11-02T12:39:07Z","timestamp":1257165547000},"page":"3939-3946","source":"Crossref","is-referenced-by-count":27,"title":["Noncovalent poly(1\u2010vinylpyrrolidone)\u2010based copolymer coating for the separation of basic proteins and lipoproteins by CE"],"prefix":"10.1002","volume":"30","author":[{"given":"Ai\u2010Jun","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Witos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"D'Ulivo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kati","family":"Vainikka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja\u2010Liisa","family":"Riekkola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00011a013"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00045a003"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(98)00485-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200111)22:19<4163::AID-ELPS4163>3.0.CO;2-9"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198108273050909"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr020466v"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600370"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048187q"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181015"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac011096y"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050896w"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700631"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500567"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305635"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200102)22:4<644::AID-ELPS644>3.0.CO;2-3"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700584"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200400091"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00085a019"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390150"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)01185-H"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00394-3"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2670(94)00557-3"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960432l"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.10.015"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)00739-8"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)01175-0"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.07.061"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700691"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700715"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800149"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.10.114"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac970041m"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00960-4"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47945-8"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104177"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103182"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112247200"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950968r"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac961231k"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.08.062"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2008.06.003"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600766"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200900395","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200900395","content-type":"unspecified","content-version":"vor","intended-application":"similarity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