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For this purpose an ion\u2010trap mass spectrometer equipped with an orthogonal ESI source operating in negative ion mode was used. An alkaline solution of ammonium acetate 5\u2009mM (pH 9) in water\/methanol (MeOH) (80:20\u2009v\/v) was delivered as coaxial sheath liquid. All experiments were performed using an uncoated fused\u2010silica capillary (90\u2009cm\u00d775\u2009\u03bcm id). The effects of variable percentages of ACN, MeOH, 2\u2010propanol, dichloromethane, along with variable concentrations of ammonium acetate were investigated for the separation of Peth. Collectively, a separation medium composed of ACN (45% v\/v), 2\u2010propanol (20% v\/v), dichloromethane (20% v\/v), MeOH (10% v\/v), water (5% v\/v), and ammonium acetate (25\u2009mM) was chosen. The estimated LOD was 0.1\u2009\u03bcM, while LOQ was 0.4\u2009\u03bcM. Within\u2010run (intra\u2010day) and between\u2010run (inter\u2010day) precision was always lower than 15%. The method proved to be robust and reliable. The MS detector allowed the simultaneous identification of several Peth homologues, and the use of an internal standard (phosphatidylbutanol) with similar electrophoretic properties of that of Peth increased quantitation effectiveness.<\/jats:p>","DOI":"10.1002\/elps.200900430","type":"journal-article","created":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T10:08:28Z","timestamp":1267438108000},"page":"1227-1233","source":"Crossref","is-referenced-by-count":37,"title":["Analysis of the alcohol biomarker phosphatidylethanol by NACE with on\u2010line ESI\u2010MS"],"prefix":"10.1002","volume":"31","author":[{"given":"Alessandro","family":"Nalesso","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Viel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Cecchetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giampietro","family":"Frison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santo Davide","family":"Ferrara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,3,26]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agl027"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.190"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1369-1600.2007.00076.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(84)90060-2"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)91507-5"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ALC.0000145687.41646.E5"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1997.tb03736.x"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(00)00291-6"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00541-0"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1998.tb03989.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ALC.0000057942.57330.E2"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00691-8"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2430"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.03.001"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-6-18"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600533"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700548"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.03.070"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800494"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.06.006"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200202)23:3<383::AID-ELPS383>3.0.CO;2-0"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.02.005"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200206)23:12<1853::AID-ELPS1853>3.0.CO;2-D"},{"key":"e_1_2_5_25_2","volume-title":"Capillary and Microchip Electrophoresis and Associated Micro\u2010Techniques","author":"Landers P. 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