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Lysozyme and BSA were chosen as model proteins to study the efficacy of <jats:italic>N<\/jats:italic>\u2010(3\u2010dimethylaminopropyl)\u2010<jats:italic>N<\/jats:italic>\u2010ethylcarbodiimide and <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide as crosslinkers. Detection of the molecular mass increase was checked by SDS\u2010PAGE. Commercially available, PVA\u2010coated capillaries showed appropriate selection, while phospho\u2010deactivated and dynamic PVA\u2010coated capillaries did not give suitable resolution. 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