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The CEC experiments were performed in fused silica capillaries (100\u2009\u03bcm\u00d730\u2009cm) packed with a 3\u2009\u03bcm cyano derivatized silica stationary phase. A laboratory\u2010made liquid junction interface was used for CEC\u2010MS coupling. The outlet capillary column was connected with an emitter tip that was positioned in front of the MS orifice. A stable electrospray was produced at nanoliter <jats:italic>per<\/jats:italic> minute flow rates applying a hydrostatic pressure (few kPa) to the interface. The coupling of packed CEC columns with mass spectrometer as detector, using a liquid junction interface, provided several advantages such as better sensitivity, low dead volume and independent control of the conditions used for CEC separation and ESI analysis. For this purpose, preliminary experiments were carried out in CEC\u2010UV to optimize the proper mobile phase for CEC analysis. Good separation efficiency was achieved for almost all compounds, using a mixture containing ACN and 25\u2009mM ammonium formate buffer at pH 3 (30:70, v\/v), as mobile phase and applying a voltage of 12\u2009kV. ESI ion\u2010trap MS detection was performed in the positive ionization mode. A spray liquid, composed by methanol\u2013water (80:20, v\/v) and 1% formic acid, was delivered at a nano\u2010flow rate of \u223c200\u2009nL\/min. Under optimized CEC\u2010ESI\u2010MS conditions, separation of the investigated drugs was performed within 13\u2009min. CEC\u2010MS and CEC\u2010MS<jats:sup>2<\/jats:sup> spectra were obtained by providing the unambiguous confirmation of these drugs in urine samples. Method precision was determined with RSDs values \u22643.3% for retention times and \u226416.3% for peak areas in both intra\u2010day and day\u2010to\u2010day experiments. LODs were established between 0.78 and 3.12\u2009ng\/mL for all compounds. Linearity was satisfactory in the concentration range of interest for all compounds (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u22650.995). The developed CEC\u2010MS method was then applied to the analysis of drugs of abuse in spiked urine samples, obtaining recovery data in the range 80\u201395%.<\/jats:p>","DOI":"10.1002\/elps.200900629","type":"journal-article","created":{"date-parts":[[2010,3,5]],"date-time":"2010-03-05T14:16:51Z","timestamp":1267798611000},"page":"1256-1263","source":"Crossref","is-referenced-by-count":29,"title":["CEC\u2010ESI ion trap MS of multiple drugs of 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