{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:09:01Z","timestamp":1761743341634,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,3,22]],"date-time":"2010-03-22T00:00:00Z","timestamp":1269216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An experiment was performed to observe protein changes in the hippocampus of zinc\u2010deficient (ZD) rats. Twenty\u2010four male weanling Wistar rats were randomly assigned to ZD (<jats:italic>n<\/jats:italic>=12) and control groups (<jats:italic>n<\/jats:italic>=12). After 4\u2010wk treatment, we used 2\u2010DE and MALDI\u2010TOF\u2010MS to analyze the proteomes of hippocampus in the two groups. One of the important differential proteins, ubiquitin C\u2010terminal hydrolase L1 (Uch\u2010L1), was confirmed by Western blot assays. The results demonstrated that compared with the controls, ZD rats had significantly reduced plasma zinc concentration and alkaline phosphatase activity. The latency period in passive avoidance performance was also significantly shorter for the ZD rats. Nine proteins were differentially expressed between the two groups. Eight of them were identified. Tubulin \u03b2 chain and voltage\u2010dependent anion channel 1 were upregulated, while mitochondrial aldehyde dehydrogenase, \u03b1\u2010enolase, dimethylarginine dimethylaminohydrolase 1, F\u2010actin capping protein \u03b1\u20102 subunit, pyruvate dehydrogenase \u03b2 and Uch\u2010L1 were downregulated, respectively. Importantly, some of the identified proteins (<jats:italic>e.g.<\/jats:italic> Uch\u2010L1) are known to be involved in cognitive impairment. Western blot analysis of hippocampus Uch\u2010L1 expression confirmed the proteomic findings. The data indicated that there may be common mechanisms or pathways in cognitive dysfunction between neurodegenerative diseases and zinc deficiency.<\/jats:p>","DOI":"10.1002\/elps.200900733","type":"journal-article","created":{"date-parts":[[2010,3,22]],"date-time":"2010-03-22T11:59:04Z","timestamp":1269259144000},"page":"1302-1310","source":"Crossref","is-referenced-by-count":9,"title":["Proteomic analysis reveals changes in the hippocampus protein pattern of rats exposed to dietary zinc deficiency"],"prefix":"10.1002","volume":"31","author":[{"given":"Jing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yugang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyun","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengtong","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1093\/jn\/125.suppl_8.2263S","volume":"125","author":"Golub M. 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