{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:32:04Z","timestamp":1762101124713,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T00:00:00Z","timestamp":1286150400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>About two\u2010thirds of the world's population is infected with <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>). This Gram\u2010negative bacterium is the most important etiological agent of chronic active type B gastritis and peptic ulcer diseases. Conventional methods such as gastric biopsy, ELISA and culture, require a long time for the determination of <jats:italic>H. pylori<\/jats:italic> infections. Moreover, the antibodies in human serum sample are capable to react immunologically with the purified <jats:italic>H. pylori<\/jats:italic> antigens immobilized on different kinds of support like magnetic nanobeads. In this study, we have developed an online immunoaffinity assay\u2010CE to determine the concentration of anti\u2010<jats:italic>H. pylori<\/jats:italic> IgG using magnetic nanobeads as a support of the immunological affinity ligands and an LIF as a detector. The separation was performed in 0.1\u2009M glycine\u2013HCl, pH 2, as the background electrolyte. The linear calibration curve to predict the concentration of <jats:italic>H. pylori<\/jats:italic>\u2010specific immunoglobulin G antibodies in serum was produced within the range of 0.12\u2013100\u2009U\/mL. The linear regression equation was <jats:italic>i<\/jats:italic>=492.86+96.03\u00d7<jats:italic>C<\/jats:italic><jats:sub><jats:italic>anti\u2010H. pylori<\/jats:italic><\/jats:sub>, with the linear regression coefficient <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>=0.999. The LOD calculated by fluorescence detection procedure was of 0.06\u2009U\/mL. The whole assay was done in no more than 35\u2009min and it was entirely automatized. The development of immunoaffinity assay\u2010CE in this study demonstrates that there is a large possibility to introduce nanotechnology in several fields with significant advantages over the classic methodologies. Our proposition comprises the diagnosis and screening field.<\/jats:p>","DOI":"10.1002\/elps.201000123","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T03:01:26Z","timestamp":1286161286000},"page":"3475-3481","source":"Crossref","is-referenced-by-count":17,"title":["Online immunoaffinity assay\u2010CE using magnetic nanobeads for the determination of anti\u2010<i>Helicobacter pylori<\/i> IgG in human serum"],"prefix":"10.1002","volume":"31","author":[{"given":"Patricia W.","family":"Stege","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Raba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germ\u00e1n A.","family":"Messina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,10,4]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"251","volume":"15","author":"Chaun H.","year":"2001","journal-title":"Gastroenterology"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001648-200103000-00023"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-136-1-200201010-00008"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00033-06"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2007.00560.x"},{"key":"e_1_2_6_7_2","first-page":"501","volume":"8","author":"van Zwet A. 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