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The analyses were performed in a bare fused\u2010silica capillary using 100\u2009mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v\/v) and 2\u2010propanol (10%, v\/v). This BGE was suitable for monitoring both off\u2010line and in\u2010capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20\u2009mM sodium phosphate, pH 7.4). 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