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Reduced expression of \u03b1\u20102 macroglobulin (AMG) and complement factor B (CFB) was detected in sera of patients with nasopharyngeal carcinoma (NPC) when pooled serum samples of the patients and those of healthy individuals were subjected to affinity isolation using immobilized champedak mannose\u2010binding lectin and analyzed by 2\u2010DE and densitometry. The AMG and CFB spots were not detected in the 2\u2010DE protein profiles when the same pooled serum samples were subjected to albumin and IgG depletion and neither were they detected when the depleted samples were analyzed by western blotting and lectin detection. Together with other acute\u2010phase response proteins that were previously reported to be altered in expression in NPC patients, AMG and CFB may serve as useful complementary biomarkers for NPC.<\/jats:p>","DOI":"10.1002\/elps.201000164","type":"journal-article","created":{"date-parts":[[2010,6,23]],"date-time":"2010-06-23T07:40:52Z","timestamp":1277278852000},"page":"2388-2395","source":"Crossref","is-referenced-by-count":28,"title":["Application of champedak mannose\u2010binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha\u20102 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"31","author":[{"given":"Ramarao","family":"Seriramalu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei Wei","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime Jacqueline","family":"Jayapalan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emida","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puteri Shafinaz","family":"Abdul\u2010Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita Zarina","family":"Bustam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan Soo\u2010Beng","family":"Khoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onn Haji","family":"Hashim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,7,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-7444-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.2002.6708"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djh056"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021659"},{"key":"e_1_2_6_6_2","first-page":"1601","volume":"14","author":"Hensley M. 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