{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T01:54:24Z","timestamp":1783043664667,"version":"3.54.6"},"reference-count":22,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2010,11,15]],"date-time":"2010-11-15T00:00:00Z","timestamp":1289779200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polyaniline nanotube (PANI\u2010NT) based films have been fabricated onto indium\u2010tin\u2010oxide (ITO) coated glass plates <jats:italic>via<\/jats:italic> electrophoretic technique. These PANI\u2010NT\/ITO electrodes have been utilized for covalent immobilization of cholesterol oxidase (ChOx) using glutaraldehyde (Glu) as cross\u2010linker. Structural, morphological and electrochemical characterization of PANI\u2010NT\/ITO electrode and ChOx\/Glu\/PANI\u2010NT\/ITO bioelectrode have been done using FT\u2010IR spectroscopy, SEM, electrochemical impedance spectroscopy and cyclic voltammetry techniques. Response studies of the ChOx\/Glu\/PANI\u2010NT\/ITO bioelectrode have been carried out using both linear sweep voltammetry and UV\u2010Visible spectrophotometry. The results of the biosensing studies reveal that this bioelectrode can be used to detect cholesterol in wide detection range of 25\u2013500\u2009mg\/dL with high sensitivity of 3.36\u2009mA\u2009mg<jats:sup>\u22121<\/jats:sup>\u2009dL and fast response time of 30\u2009s at pH 7.4. This bioelectrode exhibits very low value of Michaelis\u2013Menten constant of 1.18\u2009mM indicating enhanced interactions between cholesterol and ChOx immobilized onto this nanostructured PANI matrix.<\/jats:p>","DOI":"10.1002\/elps.201000321","type":"journal-article","created":{"date-parts":[[2010,11,15]],"date-time":"2010-11-15T02:09:48Z","timestamp":1289786988000},"page":"3754-3762","source":"Crossref","is-referenced-by-count":12,"title":["Electrophoretically deposited polyaniline nanotubes based film for cholesterol detection"],"prefix":"10.1002","volume":"31","author":[{"given":"Chetna","family":"Dhand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratima R.","family":"Solanki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj K.","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Datta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bansi D.","family":"Malhotra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11,15]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1151-2916.1996.tb08929.x"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1039\/tf9403500279"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2005.05.052"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2006.10.005"},{"key":"e_1_2_8_6_2","first-page":"438","volume":"365","author":"Tada K.","year":"2003","journal-title":"Thin Solid Films"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.synthmet.2005.07.265"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac802421h"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/elan.200403185"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/b9nr00346k"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/app.28307"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/elan.200403185"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/elan.200503415"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ces.2005.05.067"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.09.028"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbon.2008.07.028"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.08.039"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.elecom.2009.05.034"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/13\/6\/311"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.11.003"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/la702123a"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2005.10.025"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2005.10.008"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201000321","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201000321","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T08:29:14Z","timestamp":1760516954000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201000321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":22,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1002\/elps.201000321"],"URL":"https:\/\/doi.org\/10.1002\/elps.201000321","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11]]}}}