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Five out of ten tested SRAP primer combinations displayed significant polymorphisms among <jats:italic>S. japonicum<\/jats:italic> isolates from China<jats:italic>,<\/jats:italic> namely ME2\/EM1, ME4\/EM1, ME4\/EM6, ME5\/EM4 and ME5\/EM5. Analysis of the 61 <jats:italic>S. japonicum<\/jats:italic> samples from China with five SRAP primer combinations identified a total of 83 reproducible polymorphic fragments. The number of fragments using each primer combination ranged from 14 to 19, with an average of 16 polymorphic bands per primer pair, and the size of fragment ranged approximately from 100 to 1000\u2009bp. Representative\u2010specific SRAP fragments were excised from the gels, and confirmed by PCR amplification of genomic DNA using primers designed and based on the sequences of these SRAP fragments. Based on SRAP profiles, unweighted pair\u2010group method with arithmetic averages (UPGMA) dendrogram was constructed. UPGMA clustering algorithm categorized <jats:italic>S. japonicum<\/jats:italic> isolates from China into nine clades and two lineages (representing the mountainous and lake\/marshland regions). These results indicate the usefulness of the SRAP technique for revealing genetic variability among <jats:italic>S. japonicum<\/jats:italic> isolates from China, and the SRAP technique should be applicable to other living organisms.<\/jats:p>","DOI":"10.1002\/elps.201000600","type":"journal-article","created":{"date-parts":[[2011,5,3]],"date-time":"2011-05-03T06:12:21Z","timestamp":1304403141000},"page":"1364-1370","source":"Crossref","is-referenced-by-count":7,"title":["Electrophoretic detection of genetic variability among <i>Schistosoma japonicum<\/i> isolates by sequence\u2010related amplified polymorphism"],"prefix":"10.1002","volume":"32","author":[{"given":"Hui\u2010Qun","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010Hao","family":"Mo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang\u2010Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Cai","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Yun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui\u2010Qing","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Biao","family":"Weng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing\u2010Quan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,5,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70521-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/1742395307084407"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(02)00263-2"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.08.004"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1310.061423"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(02)00011-6"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5769(98)80282-1"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.78.188"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68079-3"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02443.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.05.015"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1996.0048"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(97)01044-2"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(00)01706-3"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1079\/JOH200154","volume":"75","author":"Zhu X. 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