{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:24:28Z","timestamp":1761863068586,"version":"build-2065373602"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,1,5]],"date-time":"2012-01-05T00:00:00Z","timestamp":1325721600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to adopt a general workflow for complex biological matrices with respect to a new blood\u2013brain barrier (BBB) model, a micellar electrokinetic chromatography method has been developed. The cells forming the BBB have been cultivated in a special cell growth medium in which six drugs (acetaminophen, caffeine, carbamazepine, cimetidine, indometacin and propranolol) have been dissolved and tested for their penetration properties. The results showed good to very good accordance to the reference values. Samples were directly injected onto the capillary without any pretreatment (fused silica capillary, id: 50\u2009\u03bcm, <jats:italic>L<\/jats:italic>: 48\u2009cm, <jats:italic>l<\/jats:italic>: 40\u2009cm). After method development, separations were carried out using a 60 mM borate buffer containing 200\u2009mM of SDS at 30\u2009kV, leading to an analysis time of less than 10\u2009min. Between two runs the capillary was rinsed with a mixture of equal parts of running buffer and isopropanol (70% v\/v), which proved to be very effective to remove matrix compounds. An appropriate choice of the detection wavelength (220 or 254\u2009nm) could avoid major interferences between analytes and matrix. The typical RSD% for migration times was approximately 2%, for peak areas, it ranged from 2 to 6%, which was very well acceptable for the generic method used in this study and the low concentrations investigated. The LODs ranged from 10 to 30\u2009ng\/mL.<\/jats:p>","DOI":"10.1002\/elps.201100282","type":"journal-article","created":{"date-parts":[[2012,1,5]],"date-time":"2012-01-05T22:26:02Z","timestamp":1325802362000},"page":"395-401","source":"Crossref","is-referenced-by-count":7,"title":["Fast investigations from biological matrices using CE \u2013 Test of a blood\u2013brain barrier model"],"prefix":"10.1002","volume":"33","author":[{"given":"Sascha","family":"K\u00fchne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Untucht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Steinert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"W\u00e4tzig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(77)80008-0"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd725"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015913817221"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2368"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016462205267"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2008.12.002"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1952"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-5949.2010.00183.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280802282934"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0197-0186(85)90002-6"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00829-9"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)02980-9"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-004-1377-8"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-007-9195-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0911-6"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.06.004"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900576"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(93)80114-G"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700016"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150191603"},{"volume-title":"The Hydrophobic Effect: Formation of Micelles and Biological Membranes","year":"1980","author":"Tanford C.","key":"e_1_2_6_22_2"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.01.058"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410191"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.04.037"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.11.071"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.11.007"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410208"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900768"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500586"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.06.015"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600537"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410185"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201000398"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200290009"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.37.707"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)80154-Z"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181026"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990801)20:12<2379::AID-ELPS2379>3.0.CO;2-4"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02467701"},{"volume-title":"Arzneibuch\u2010Kommentar: Wissenschaftliche Erl\u00e4uterungen zum Europ\u00e4ischen Arzneibuch und zum Deutschen Arzneibuch","year":"2004","author":"B\u00f6hme H.","key":"e_1_2_6_41_2"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.05.012"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci900384c"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(04)03065-X"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-007-9502-2"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0223-5234(01)01269-7"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm00398a028"},{"key":"e_1_2_6_48_2","unstructured":"Anon. Agilent Capillary Electrophoresis System User's Guide Waldbronn2000."},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201000587"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00045a003"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201000245"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900015"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0415859"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700678"},{"key":"e_1_2_6_55_2","unstructured":"European Pharmacopoeia 2008 Dt. Apotheker\u2010Verl. Stuttgart"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.09.011"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci034205d"},{"key":"e_1_2_6_58_2","first-page":"56P","volume":"505","author":"Dolman D. E. M.","year":"1997","journal-title":"J. Physiol."},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(96)01883-3"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)90276-3"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.40.2510"},{"key":"e_1_2_6_62_2","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0022-3565(25)24549-9","volume":"272","author":"van Belle K.","year":"1995","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.35.95"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015935031933"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201100282","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201100282","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.201100282","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201100282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:42:23Z","timestamp":1760542943000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201100282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":63,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1002\/elps.201100282"],"URL":"https:\/\/doi.org\/10.1002\/elps.201100282","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2012,1]]}}}