{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T05:23:02Z","timestamp":1788067382643,"version":"build-2784847793"},"reference-count":113,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2011,8,17]],"date-time":"2011-08-17T00:00:00Z","timestamp":1313539200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We barcoded 25 in vitro isolates (representing 92 samples) of <jats:italic>Giardia duodenalis<\/jats:italic> from humans and other animals, which have been assembled by the Upcroft team at the Queensland Institute of Medical Research over a period of almost three decades. We used mutation scanning\u2010coupled sequencing of loci in the triosephosphate isomerase, glutamate dehydrogenase and \u03b2\u2010giardin genes, combined with phylogenetic analysis, to genetically characterise them. Specifically, the isolates (<jats:italic>n<\/jats:italic>=14) of <jats:italic>G<\/jats:italic>. <jats:italic>duodenalis<\/jats:italic> from humans from Australia (AD113; BRIS\/83\/HEPU\/106; BRIS\/87\/HEPU\/713; BRIS\/89\/HEPU\/1003; BRIS\/92\/HEPU\/1541; BRIS\/92\/HEPU\/1590; BRIS\/92\/HEPU\/2443; BRIS\/93\/HEPU\/1706), Malaysia (KL\/92\/IMR\/1106) and Afghanistan (WB), a cat from Australia (BAC2), a sheep from Canada (OAS1) and a sulphur\u2010crested cockatoo from Australia (BRIS\/95\/HEPU\/2041) represented assemblage A (sub\u2010assemblage AI\u20101, AI\u20102 or AII\u20102); isolates (<jats:italic>n<\/jats:italic>=10) from humans from Australia (BRIS\/91\/HEPU\/1279; BRIS\/92\/HEPU\/2342; BRIS\/92\/HEPU\/2348; BRIS\/93\/HEPU\/1638; BRIS\/93\/HEPU\/1653; BRIS\/93\/HEPU\/1705; BRIS\/93\/HEPU\/1718; BRIS\/93\/HEPU\/1727), Papua New Guinea (BRIS\/92\/HEPU\/1487) and Canada (H7) represented assemblage B (sub\u2010assemblage BIV) and an isolate from cattle from Australia (BRIS\/92\/HEPU\/1709) had a match to assemblage E. Isolate BRIS\/90\/HEPU\/1229 from a human from Australia was shown to represent a mixed population of assemblages A and B. These barcoded isolates (including stocks and derived lines) now allow direct comparisons of experimental data among laboratories and represent a massive resource for transcriptomic, proteomic, metabolic and functional genomic studies using advanced molecular technologies. (Nucleotide sequences reported in this paper are available in the GenBank database under accession nos. JN204442\u2013JN204461.)<\/jats:p>","DOI":"10.1002\/elps.201100283","type":"journal-article","created":{"date-parts":[[2011,8,17]],"date-time":"2011-08-17T02:40:03Z","timestamp":1313548803000},"page":"2075-2090","source":"Crossref","is-referenced-by-count":18,"title":["Barcoding of <i>Giardia duodenalis<\/i> isolates and derived lines from an established cryobank by a mutation scanning\u2010based approach"],"prefix":"10.1002","volume":"32","author":[{"given":"Matthew J.","family":"Nolan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron R.","family":"Jex","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqui A.","family":"Upcroft","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Upcroft","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,8,17]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"2","volume-title":"Parasitic Protozoa","author":"Kulda J.","year":"1978"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2010.02.015"},{"key":"e_1_2_6_4_2","first-page":"209","volume":"59","author":"Rendtorff R. 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