{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:09:04Z","timestamp":1772971744915,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T00:00:00Z","timestamp":1335139200000},"content-version":"vor","delay-in-days":53,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>A capillary zone electrophoresis\u2010based method to evaluate the cytotoxicity of substances to Caco\u20102 cells was established. The estimation of the injected cell number (500\u20135000) and the minor effect of injection condition on cytotoxicity determination were investigated. Caco\u20102 cells the best model of the intestinal absorptive epithelium, were treated with substances and then stained with Trypan Blue and fixed with paraformaldehyde. The treated Caco\u20102 cells were detected simultaneously at 590 nm and 214 nm, and the absorbance ratio of the two wavelengths (<jats:italic>R<\/jats:italic><jats:sub>590\/214<\/jats:sub>) can reflect simultaneously the loss of cell membrane integrity and the degradation\/leak of intracellular components and indicate the cytotoxicity of substances. The cytotoxicity of the four substances sodium sulfite (Na<jats:sub>2<\/jats:sub>SO<jats:sub>3<\/jats:sub>), methyl mercury (<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>e<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>g), paclitaxel (<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>), and cadmium chloride (<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>d<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>) were determined and compared. There was no obvious cytotoxicity caused by 20 \u03bcM <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>a<jats:sub>2<\/jats:sub><jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content><jats:sub>3<\/jats:sub> for 24 h treatment, and the toxicity of the other three toxicants was sequenced as: <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>dCl<jats:sub>2<\/jats:sub> &gt; <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>e<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>g&gt; <jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>. The results are in good agreement with the references and the conventional Trypan Blue exclusion counting assay.<\/jats:p>","DOI":"10.1002\/elps.201100382","type":"journal-article","created":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T04:42:29Z","timestamp":1335156149000},"page":"834-840","source":"Crossref","is-referenced-by-count":6,"title":["Capillary zone electrophoresis\u2010based cytotoxicity analysis of Caco\u20102 cells"],"prefix":"10.1002","volume":"33","author":[{"given":"Lu","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Science, Beijing Institute of Technology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Qu","sequence":"additional","affiliation":[{"name":"School of Life Science, Beijing Institute of Technology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiling","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Life Science, 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