{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:42:27Z","timestamp":1760575347969,"version":"build-2065373602"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T00:00:00Z","timestamp":1335484800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>The analysis of glycoprotein isoforms is of high interest in the biomedical field and clinical chemistry. Many studies have demonstrated that some glycoprotein isoforms could serve as biomarkers for several major diseases, such as cancers and vascular diseases, among others. Capillary zone electrophoresis (<jats:styled-content style=\"fixed-case\">CZE<\/jats:styled-content>) is a well\u2010established technique to separate glycoprotein isoforms, however, it suffers from limited sensitivity when <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>is detection is used. On the other hand, with laser\u2010induced fluorescence (<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>) detection, derivatization reaction to render the proteins fluorescent can destroy the resolution of the isoforms. In this work, a derivatization procedure through the thiol groups of glycoproteins using either 5\u2010(iodoacetamide) fluorescein (5<jats:bold>\u2010<\/jats:bold><jats:styled-content style=\"fixed-case\">IAF<\/jats:styled-content>) or <jats:styled-content style=\"fixed-case\">BODIPY<\/jats:styled-content> iodoacetamide is presented with the model protein of alpha\u20101\u2010acid glycoprotein (<jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content>). The derivatization process presented enabled high\u2010resolution analysis of <jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content> isoforms by <jats:styled-content style=\"fixed-case\">CZE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>. The derivatization procedure was successfully applied to label <jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content> from samples of serum and secretome of artery tissue, enabling the separation of the <jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content> isoforms by <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> in natural samples at different concentration levels.<\/jats:p>","DOI":"10.1002\/elps.201100473","type":"journal-article","created":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T05:28:29Z","timestamp":1335504509000},"page":"1113-1119","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of alpha\u20101\u2010acid glycoprotein isoforms using <scp>CE<\/scp>\u2010<scp>LIF<\/scp> with fluorescent thiol derivatization"],"prefix":"10.1002","volume":"33","author":[{"given":"Ra\u00fal","family":"Garrido\u2010Medina","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry (CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Puerta","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry (CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuly","family":"Rivera\u2010Monroy","sequence":"additional","affiliation":[{"name":"Horvath Laboratory of Bioseparation Sciences, University of Debrecen Debrecen  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes","family":"de Frutos","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry (CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andras","family":"Guttman","sequence":"additional","affiliation":[{"name":"Horvath Laboratory of 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