{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T09:06:28Z","timestamp":1773479188697,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,8,7]],"date-time":"2012-08-07T00:00:00Z","timestamp":1344297600000},"content-version":"vor","delay-in-days":67,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>A NACE method with laser\u2010induced fluorescence detection was modified for sensitive detection of 4 tetracyclines (TCs) in biological samples and feeds. The changes in injection mode, injection times, id of capillary, excitation wavelength, and the use of surfactant and sample stacking technique all contributed to improved LODs of TCs to sub\u2010ng\/mL level. With the optimized conditions, the instrumental LODs could reach 1.33 ng\/mL for chlorotetracycline (CTC) and 13.3 ng\/mL for TC, oxytetracycline (OTC), and doxycycline (DC), an improvement of 10\u2013100\u2010fold over past studies. A simple SPE procedure was further developed for the extraction and concentration of TCs in plasma, urine, feed, and milk. Taken together, the instrumental LOD and feasible SPE concentration factors the overall LODs for CTC could reach 65 pg\/mL in feed and milk and 260 pg\/mL in plasma and urine. Detection limits for TC, OTC, and DC at sub\u2010ng\/mL level were also achieved. The modified CE\u2010LIF method was found to be less complicated and more sensitive than the best current methods using UV or LIF detection, and has been applied successfully to assess oral absorption of DC in swine and chickens and to confirm suspected TC\u2010positive bovine serum samples.<\/jats:p>","DOI":"10.1002\/elps.201100485","type":"journal-article","created":{"date-parts":[[2012,8,7]],"date-time":"2012-08-07T06:19:52Z","timestamp":1344320392000},"page":"1679-1682","source":"Crossref","is-referenced-by-count":23,"title":["Improved nonaqueous capillary electrophoresis for tetracyclines at subparts per billion level"],"prefix":"10.1002","volume":"33","author":[{"given":"Tzu\u2010Yi","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine College of Veterinary Medicine National Chung Hsing University Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas W.","family":"Vickroy","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences College of Veterinary Medicine University of Florida Gainesville FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jui\u2010Hung","family":"Shien","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine College of Veterinary Medicine National Chung Hsing University Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Chung","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine College of Veterinary Medicine National Chung Hsing University Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8,7]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200108)22:13<2775::AID-ELPS2775>3.0.CO;2-2"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.01.105"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(94)00434-7"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01290-9"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.08.015"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.08.004"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00260-1"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.08.012"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.01.036"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600614"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2006.02.048"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00421-4"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.01.084"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600671107"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700276"},{"key":"e_1_2_4_17_1","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1093\/jaoac\/78.6.1369","volume":"78","author":"Chen C. 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