{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:31:08Z","timestamp":1791927068073,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,7]]},"abstract":"Asymmetric dimethylarginine (ADMA<\/jats:styled-content>) is an inhibitor of nitric oxide synthase and a risk factor for cardiovascular events. We have developed a new derivatization method to enable baseline separation of the regio\u2010isomers, ADMA<\/jats:styled-content>, and symmetric dimethylarginine (SDMA<\/jats:styled-content>), within 15 min on a C<\/jats:styled-content>18 reverse phase column. Reacting naphthalene\u20102,3\u2010dicarboxaldehyde with ADMA<\/jats:styled-content> and SDMA<\/jats:styled-content> in the presence of 2\u2010mercaptoethanol produces corresponding 2,3\u2010dihydro\u2010benzo[f]isoindol\u20101\u2010ones that are more stable than previously reported ortho\u2010phthaldialdehyde and 2\u2010mercaptoethanol derivatives. LC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> quantitation of these derivatives can be used to determine ADMA<\/jats:styled-content> and SDMA<\/jats:styled-content> concentrations in the plasma of patients to receive on\u2010pump coronary artery bypass grafting (CABG)<\/jats:styled-content> surgery. The LOD, LOQ and lower LOQ (LLOQ) of this method were determined to be 2.6, 8.7, and 25 nM for ADMA<\/jats:styled-content>, and 2.5, 8.3, and 25 nM for SDMA<\/jats:styled-content>, respectively, with consumption of only 50 \u03bcL of plasma. The relative standard deviations and relative errors of the intraday and interday determinations, as measurements of reproducibility and accuracy, are all within 15%. The ADMA<\/jats:styled-content> and SDMA<\/jats:styled-content> concentrations in patient plasma are 298.1 \u00b1 11.2 nM (mean \u00b1 S.E.M.<\/jats:styled-content>, n<\/jats:italic> = 123) and 457.7 \u00b1 19.8 nM (mean \u00b1 S.E.M.<\/jats:styled-content>, n<\/jats:italic> = 123), respectively. Upon unblinding of our clinical trial, these predetermined values might explain patient clinical outcomes associated with on\u2010pump CABG<\/jats:styled-content> surgery, as ADMA<\/jats:styled-content> is known to inhibit nitric oxide production. Furthermore, this derivatization reaction in conjunction with LC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> analysis may open a venue to explore alternative chemical labeling modes for LC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> applications, such as analysis of other amino acids, metabolites, and peptides containing primary amine group(s).<\/jats:p>","DOI":"10.1002\/elps.201100536","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T07:26:45Z","timestamp":1340868405000},"page":"1911-1920","source":"Crossref","is-referenced-by-count":12,"title":["A new derivatization method coupled with LC<\/scp>\u2010MS<\/scp>\/MS<\/scp> to enable baseline separation and quantification of dimethylarginines in human plasma from patients to receive on\u2010pump CABG<\/scp> surgery"],"prefix":"10.1002","volume":"33","author":[{"given":"Yu","family":"Hui","sequence":"first","affiliation":[{"name":"Department of Chemistry University of British Colombia Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of British Colombia Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo\u2010oll","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of British Colombia Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of British Colombia Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Ansley","sequence":"additional","affiliation":[{"name":"Department of 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