{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:38:06Z","timestamp":1782977886641,"version":"3.54.5"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p>Intrauterine growth restriction (<jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content>) is defined as a condition in which the fetus does not reach its genetically given growth potential, resulting in low birth weight. <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> is an important cause of perinatal morbidity and mortality, thus contributing substantially to medically indicated preterm birth in order to prevent fetal death. We subjected umbilical cord blood serum samples either belonging to the <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> group (<jats:italic>n<\/jats:italic> = 15) or to the control group (<jats:italic>n<\/jats:italic> = 15) to fractionation by affinity chromatography using a bead system with hydrophobic interaction capabilities. So prepared protein mixtures were analyzed by <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> mass spectrometric profiling. The six best differentiating ion signals at <jats:italic>m\/z<\/jats:italic> 8205, <jats:italic>m\/z<\/jats:italic> 8766, <jats:italic>m\/z<\/jats:italic> 13 945, <jats:italic>m\/z<\/jats:italic> 15 129, <jats:italic>m\/z<\/jats:italic> 15 308, and <jats:italic>m\/z<\/jats:italic> 16 001 were collectively assigned as <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> proteome signature. Separation confidence of our <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> proteome signature reached a sensitivity of 0.87 and a specificity of 0.93. Assignment of ion signals in the mass spectra to specific proteins was substantiated by <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> in conjunction with peptide mass fingerprint analysis of cord blood serum proteins. One constituent of this proteome signature, apolipoprotein <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010III<jats:sub>0<\/jats:sub>, a derivative lacking glycosylation, has been found more abundant in the <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> cord blood serum samples, irrespective of gestational age. Hence, we suggest apolipoprotein <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010III<jats:sub>0<\/jats:sub> as potential key\u2010marker of the here proposed <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> proteome signature, as it is a very low\u2010density lipoprotein (<jats:styled-content style=\"fixed-case\">VLDL<\/jats:styled-content>) and high\u2010density lipoprotein (<jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>) member and as such involved in triglyceride metabolism that itself is discussed as being of importance in <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content> pathogenesis. Our results indicate that subtle alterations in protein glycosylation need to be considered for improving our understanding of the pathomechanisms in <jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/elps.201200001","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T07:26:45Z","timestamp":1340868405000},"page":"1981-1993","source":"Crossref","is-referenced-by-count":22,"title":["A proteome signature for intrauterine growth restriction derived from multifactorial analysis of mass spectrometry\u2010based cord blood serum profiling"],"prefix":"10.1002","volume":"33","author":[{"given":"Manja","family":"W\u00f6lter","sequence":"first","affiliation":[{"name":"Proteome Center Rostock Medical Faculty and Natural Science Faculty University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"R\u00f6wer","sequence":"additional","affiliation":[{"name":"Proteome Center Rostock Medical Faculty and Natural Science Faculty University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Koy","sequence":"additional","affiliation":[{"name":"Proteome Center Rostock Medical Faculty and Natural Science Faculty University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toralf","family":"Reimer","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Medical Faculty University of Rostock Clinic Suedstadt  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Rath","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Medical Faculty RWTH Aachen University Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Pecks","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Medical Faculty RWTH Aachen University Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael O.","family":"Glocker","sequence":"additional","affiliation":[{"name":"Proteome Center Rostock Medical Faculty and Natural Science Faculty University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.107607"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60279-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.10.813"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7292(00)80016-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7292(00)90000-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/S1701-2163(16)34956-8","volume":"33","author":"al Riyami N.","year":"2011","journal-title":"J. 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