{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:02:48Z","timestamp":1762340568850,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2012,7,16]],"date-time":"2012-07-16T00:00:00Z","timestamp":1342396800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p>A chiral capillary electrophoretic method with nearly full p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> window was explored for the separation and determination of <jats:sc>dl<\/jats:sc>\u2010penicillamine. A facile one\u2010pot labeling technique was coupled in the method for introduction of chromophore and charge groups onto the analytes to facilitate the electromigration and sensitive detection. By using simply a cost\u2010effective neutral \u03b2\u2010cyclodextrin as chiral selector, baseline separation of the <jats:sc>dl<\/jats:sc>\u2010penicillamine was achieved from p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> 2.0 to over p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> 10. Quantification of standard <jats:sc>d<\/jats:sc>\u2010 and <jats:sc>l<\/jats:sc>\u2010penicillamines was demonstrated by taking p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> 4.5, 7.4, and 9.7 as the representatives of acidic, neutral, and basic conditions. The working curves were constructed between peak area and concentration, having linear ranges of 8.56\u20138.56 \u00d7 10<jats:sup>2<\/jats:sup> \u03bcg\/mL for pH 4.5 and 8.56\u20131.71 \u00d7 10<jats:sup>3<\/jats:sup> \u03bcg\/mL for pH 7.4 and 9.7, with correlation coefficients all better than 0.999. The limit of detection (S\/N = 3) was 2.58 \u03bcg\/mL in acidic and neutral conditions or 1.41 \u03bcg\/mL in basic condition. The method was further validated by assaying the commercial penicillamine tablets, applicable to quantification of the effective enantiomer and the trace impurity of <jats:sc>l<\/jats:sc>\u2010penicillamine at a content of down to 0.2, 0.6, and 2.0% for pH 9.7, 4.5, and 7.4, respectively. The recovery determined by spiking technique was in a range from 93.1 to 105 %. The method is easily extendable to the analysis of other chiral amines or amino acids.<\/jats:p>","DOI":"10.1002\/elps.201200046","type":"journal-article","created":{"date-parts":[[2012,7,16]],"date-time":"2012-07-16T10:11:14Z","timestamp":1342433474000},"page":"2056-2063","source":"Crossref","is-referenced-by-count":28,"title":["Separation and determination of chiral composition in penicillamine tablets by capillary electrophoresis in a broad p<scp>H<\/scp> range"],"prefix":"10.1002","volume":"33","author":[{"given":"Lijuan","family":"Song","sequence":"first","affiliation":[{"name":"Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenpeng","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences  Beijing 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