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To evaluate the effect of weight loss on AMPK<\/jats:styled-content>, sera from 24 patients were collected before and after intragastric balloon insertion over a 16\u2010week follow\u2010up period. Compared to baseline, all patients showed postoperatively an increase of AMPK<\/jats:styled-content>. Patients with ischemic heart, with inflammatory bowel disease, with chronic undernutrition or with hepatic diseases were examined. Compared to control subjects, the majority of them showed a significant increase of AMPK<\/jats:styled-content>. These results suggest that serum AMPK<\/jats:styled-content> may have a potential for diagnosis of several metabolic diseases. 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