{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T21:25:43Z","timestamp":1761427543168,"version":"build-2065373602"},"reference-count":86,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T00:00:00Z","timestamp":1354579200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Transmissible spongiform encephalopathies are a group of fatal neurodegenerative diseases with long incubation time. This group includes <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>reutzfeld\u2010<jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>akob disease, kuru, scrapie, chronic wasting disease, and bovine spongiform encephalopathy. Sensitive and specific detection of abnormal prion protein as \u201ca source agent\u201d of the above\u2010mentioned diseases in blood could provide a diagnostic test or a screening assay for animal and human prion protein diseases diagnostics. Therefore, diagnostic tests for prion protein diseases represent unique challenge requiring development of novel assays exploiting properties of prion protein complex. Presently, diagnostic methods such as protein misfolding cyclic amplification, conformation\u2010dependent immunoassay, dissociation\u2010enhanced lanthanide fluorescent immunoassay, fluorescence correlation spectroscopy, and\/or flow microbead immunoassay are used for abnormal prion protein (PrP<jats:sup>Sc<\/jats:sup>) detection. On the other hand, using of CE for PrP<jats:sup>Sc<\/jats:sup> detection in body fluids is an attractive alternative; it has been already applied for the blood samples of infected sheep, elk, chimpanzee, as well as humans. In this review, assays for prion protein detection are summarized with special attention to capillary electromigration based techniques, such as CE, CIEF, and\/or CGE. The potential of the miniaturized and integrated lab\u2010on\u2010chip devices is highlighted, emphasizing recent advances of this field in the proteomic analysis.<\/jats:p>","DOI":"10.1002\/elps.201200208","type":"journal-article","created":{"date-parts":[[2012,9,19]],"date-time":"2012-09-19T08:14:06Z","timestamp":1348042446000},"page":"3644-3652","source":"Crossref","is-referenced-by-count":5,"title":["Capillary electromigration based techniques in diagnostics of prion protein caused diseases"],"prefix":"10.1002","volume":"33","author":[{"given":"Pavlina","family":"Sobrova","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno  Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marketa","family":"Ryvolova","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno  Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vojtech","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno  Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rene","family":"Kizek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno  Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-559"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13363"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.10962"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1675487"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/pri.3.4.9980"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050870"},{"key":"e_1_2_6_8_1","first-page":"299","volume-title":"Annual Review of Physical Chemistry","author":"Millhauser G. 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