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Experimental parameters including sample matrix, surfactant concentration, injection length and concentration of high\u2010conductivity buffer, and sample electrokinetic injection time were optimized for electrophoretic enrichment and separation processes. Under the optimal conditions, the detection sensitivity of cotinine was enhanced by about 5000\u2010fold using <jats:styled-content style=\"fixed-case\">CSEI<\/jats:styled-content>\u2010sweeping <jats:styled-content style=\"fixed-case\">MEKC<\/jats:styled-content> compared to normal <jats:styled-content style=\"fixed-case\">MEKC<\/jats:styled-content>. The limit of detection for cotinine was found to be 0.2 ng\/mL using ultraviolet absorbance detection. 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