{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:29:15Z","timestamp":1792621755466,"version":"4.5.0"},"reference-count":80,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T00:00:00Z","timestamp":1362960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells is used for cancer diagnosis and prognosis, while <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> analysis or enumeration of nucleated red blood cells is useful for prenatal diagnosis or hypoxic anemia, and that of circulating stem cells to diagnose cancer metastasis. Isolation of these cells and their downstream analyses can provide significant information such as the origin and characteristics of a disease. Novel approaches based on microfluidics have many advantages, including the continuous process and integration with other components for analysis. For these reasons, a variety of microfluidic devices have been developed to isolate and characterize rare cells. In this article, we review several microfluidic devices, with a focus on affinity\u2010based isolation (e.g. antigen\u2010antibody reaction) and label\u2010free separation (DEP and hydrophoresis).<\/jats:p>","DOI":"10.1002\/elps.201200417","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T04:11:34Z","timestamp":1361506294000},"page":"1028-1041","source":"Crossref","is-referenced-by-count":60,"title":["Microfluidic devices for the isolation of circulating rare cells: A focus on affinity\u2010based, dielectrophoresis, and hydrophoresis"],"prefix":"10.1002","volume":"34","author":[{"given":"Kyung\u2010A","family":"Hyun","sequence":"first","affiliation":[{"name":"School of Mechanical Engineering, Yonsei University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo\u2010Il","family":"Jung","sequence":"additional","affiliation":[{"name":"School of Mechanical Engineering, Yonsei University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anchem.111808.073610"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4048\/jbc.2010.13.2.125"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.05.007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc5932"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1182\/blood.V47.6.1031.1031","volume":"47","author":"Richman C.M.","year":"1976","journal-title":"Blood"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1182\/blood.V41.1.67.67","volume":"41","author":"Chervenick P. A.","year":"1973","journal-title":"Blood"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.09.047"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmmm.2008.12.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000135655.52088.c5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043814"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1150224"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1142"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac102975g"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05063"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06385"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1012539107"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200901668"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201005853"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/b704602b"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/la9045572"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8015022"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac103172y"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2007.12.004"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac802092j"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9012072"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-130260"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc40072c"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2012.06.016"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1700065"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.3456626"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/es900078z"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-010-0370-9"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/00032719.2011.633189"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2011.08.013"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00345j"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/182581"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1088\/1742-6596\/34\/1\/182"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.860"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/0022-3727\/27\/12\/030"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78144-X"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac048196z"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/152581699319939"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac981250p"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800373"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/b717043b"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc21273k"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1039\/b819267g"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201000625"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2743-7"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2011.03.042"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac971274g"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800366"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-007-9130-y"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1109\/JSEN.2010.2044787"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4731647"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1039\/b920590j"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708760104"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-007-9061-7"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2009.44"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1039\/b710054j"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3721-9"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.05.064"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1039\/b922301k"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.01.065"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.05.036"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac200550d"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2010.07.054"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1039\/B813952K"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9005765"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00109k"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2012.07.021"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00633e"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.3576780"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094567"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605967103"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2079"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602310"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc40065k"},{"key":"e_1_2_7_80_1","doi-asserted-by":"crossref","unstructured":"Gorges T. M. Tinhofer I. Drosch M. R\u00f6se L. Zollner T. M. Krahn T. vonAhsen Oliver. BMC Cancer2012 DOI:10.1186\/1471\u20132407\u201012\u2013178.","DOI":"10.1186\/1471-2407-12-178"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2005.12.037"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201200417","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201200417","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:34:41Z","timestamp":1760524481000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201200417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,11]]},"references-count":80,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1002\/elps.201200417"],"URL":"https:\/\/doi.org\/10.1002\/elps.201200417","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,11]]}}}