{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:46:11Z","timestamp":1777549571804,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T00:00:00Z","timestamp":1363132800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,3]]},"abstract":"From 35 species of marine fishes (n<\/jats:italic> = 327) from the South China Sea, 237 nematode larvae were collected and identified morphologically as Anisakis<\/jats:italic>. Genomic DNA<\/jats:styled-content> was isolated from each larva and subjected to PCR<\/jats:styled-content>\u2010based RFLP<\/jats:styled-content> and targeted sequencing of a nuclear ribosomal DNA<\/jats:styled-content> region between the 3\u2032\u2010end of the small subunit and 5\u2032\u2010end of the large subunit of the rRNA<\/jats:styled-content> genes (= internal transcribed spacers, ITS<\/jats:styled-content>+). Four different RFLP<\/jats:styled-content> profile combinations (sets) were detected for all restriction endonucleases (Hinf<\/jats:italic>I, Hha<\/jats:italic>I, and Taq<\/jats:italic>I), of which three were characteristic of Anisakis typica<\/jats:italic>, A. pegreffii,<\/jats:italic> and A. physeteris<\/jats:italic>, respectively. One profile set (for sample CA<\/jats:styled-content>\u20102012) was linked to an ITS<\/jats:styled-content>+ sequence that was identical to a previously published sequence of Anisakis<\/jats:italic> sp. (sample HC<\/jats:styled-content>\u20102005; originating from the African shelf) and another sequence (PH<\/jats:styled-content>\u20102010; Madeira, Portugal). Phylogenetic analysis was carried out using the ITS<\/jats:styled-content>+ sequence data from this study and reference sequences from the GenBank database. Neighbor joining and maximum parsimony trees displayed three clades. Clades I and II included nine described species of Anisakis<\/jats:italic>, including all type I and type II larvae; clade III represented some undescribed species of Anisakis<\/jats:italic>. Morphological comparison showed that Anisakis<\/jats:italic> sp. CA\u20102012 was distinct from type I and type II larvae based on its tail shape and ratio of tail length to body length. The phylogenetic analysis and morphological characters suggest that Anisakis<\/jats:italic> sp. CA\u20102012 represents a new record, now called Anisakis<\/jats:italic> type III larvae.<\/jats:p>","DOI":"10.1002\/elps.201200493","type":"journal-article","created":{"date-parts":[[2013,1,20]],"date-time":"2013-01-20T04:34:43Z","timestamp":1358656483000},"page":"888-894","source":"Crossref","is-referenced-by-count":19,"title":["Identification and genetic characterization of A<\/scp>nisakis<\/i> larvae from marine fishes in the S<\/scp>outh C<\/scp>hina S<\/scp>ea using an electrophoretic\u2010guided approach"],"prefix":"10.1002","volume":"34","author":[{"given":"Luping","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Life Science Hebei Normal University Shijiazhuang Hebei Province P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojie","family":"Du","sequence":"additional","affiliation":[{"name":"College of Life Science Hebei Normal University Shijiazhuang Hebei Province P. R. 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