{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:17:36Z","timestamp":1773289056263,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T00:00:00Z","timestamp":1366675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,6]]},"abstract":"The one\u2010carbon cycle is composed of four major biologically important molecules: methionine (l<\/jats:sc>\u2010M<\/jats:styled-content>et), S<\/jats:styled-content><\/jats:italic>\u2010adenosylmethionine (SAM<\/jats:styled-content>), S<\/jats:styled-content><\/jats:italic>\u2010adenosylhomocysteine (SAH<\/jats:styled-content>), and homocysteine (H<\/jats:styled-content>cy). In addition to these key metabolites, there are multiple enzymes, vitamins, and cofactors that play essential roles in the cascade of the biochemical reactions that convert one metabolite into another in the cycle. Simultaneous quantitative measurement of four major metabolites can be used to detect possible aberrations in this vital cycle. Abnormalities in the one\u2010carbon cycle might lead to hyper\u2010 or hypomethylation, homocystinemia, liver dysfunction, and accumulation of white\u2010matter hyperintensities in the human brain. Previously published methods describe evaluation of several components of the one\u2010carbon cycle, but none to our knowledge demonstrated simultaneous measurement of all four key molecules (l<\/jats:sc>\u2010M<\/jats:styled-content>et, SAM<\/jats:styled-content>, SAH<\/jats:styled-content>, and H<\/jats:styled-content>cy). We describe a novel analytical method suitable for simultaneous identification and quantification of l<\/jats:sc>\u2010M<\/jats:styled-content>et, SAM<\/jats:styled-content>, SAH<\/jats:styled-content>, and H<\/jats:styled-content>cy with LC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content>. Moreover, we tested this method to identify these metabolites in human plasma collected from patients with multiple sclerosis and healthy individuals. In a pilot feasibility study, our results indicate that patients with multiple sclerosis showed abnormalities in the one\u2010carbon cycle.<\/jats:p>","DOI":"10.1002\/elps.201200536","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T21:05:18Z","timestamp":1361221518000},"page":"1710-1716","source":"Crossref","is-referenced-by-count":18,"title":["LC<\/scp>\u2010MS<\/scp>\/MS<\/scp> identification of the one\u2010carbon cycle metabolites in human plasma"],"prefix":"10.1002","volume":"34","author":[{"given":"Lidia A.","family":"Gardner","sequence":"first","affiliation":[{"name":"Research Service Veterans Administration Medical Center Memphis TN USA"},{"name":"Department of Neurology University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic M.","family":"Desiderio","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Tennessee Health Science Center Memphis TN USA"},{"name":"Charles B. Stout Neuroscience Research Laboratory University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chassidy J.","family":"Groover","sequence":"additional","affiliation":[{"name":"Research Service Veterans Administration Medical Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Hartzes","sequence":"additional","affiliation":[{"name":"Research Service Veterans Administration Medical Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles R.","family":"Yates","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey R.","family":"Zucker\u2010Levin","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard","family":"Bloom","sequence":"additional","affiliation":[{"name":"Cytopathology Laboratory Vanderbilt University Medical Center Nashville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Levin","sequence":"additional","affiliation":[{"name":"Research Service Veterans Administration Medical Center Memphis TN USA"},{"name":"Department of Neurology University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-18-06920.2000"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/13.3.656"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.2839"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.10.1650"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.046995"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.026252"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1093\/clinchem\/48.9.1539","volume":"48","author":"Hanson N. 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