{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:51:16Z","timestamp":1760575876366,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T00:00:00Z","timestamp":1363132800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:p>Abnormalities in plasma von Willebrand factor (v<jats:styled-content style=\"fixed-case\">WF<\/jats:styled-content>) concentration and function result in von Willebrand disease (v<jats:styled-content style=\"fixed-case\">WD<\/jats:styled-content>). The diagnosis requires a battery of tests such as screening procedures, confirmatory tests, phenotypic characterization, and genotyping. The phenotypic testing (multimer pattern analysis) is important in order to subclassify the hereditary and the acquired forms of v<jats:styled-content style=\"fixed-case\">WD<\/jats:styled-content>. Only few laboratories are skilled to perform this analysis. The extreme range of protein size from 250 kDa monomer to over 20\u00a0000 kDa multimers requires a time\u2010consuming procedure (3\u20134 days) and presents many technical difficulties. To standardize the method and to overcome technical difficulties, we developed a rapid and sensitive semi\u2010automated method to visualize the multimeric structure of v<jats:styled-content style=\"fixed-case\">WF<\/jats:styled-content>. The semi\u2010automated method we present performs the electrophoresis of patient's plasma in 120 min on a precast gel. Gels are suitable for the <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>26 Interlab instrumentation. After gel blotting, the method allows visualization of the v<jats:styled-content style=\"fixed-case\">WF<\/jats:styled-content> multimer pattern directly on the membrane. We reduced the time required from 72 to 8 h and we propose this test for the first level screening of v<jats:styled-content style=\"fixed-case\">WF<\/jats:styled-content> multimer deficiency.<\/jats:p>","DOI":"10.1002\/elps.201200538","type":"journal-article","created":{"date-parts":[[2013,1,20]],"date-time":"2013-01-20T04:34:45Z","timestamp":1358656485000},"page":"818-824","source":"Crossref","is-referenced-by-count":2,"title":["von <scp>W<\/scp>illebrand <scp>F<\/scp>actor multimers profiling with a semi\u2010automated system"],"prefix":"10.1002","volume":"34","author":[{"given":"Fabio","family":"Pasotti","sequence":"first","affiliation":[{"name":"Department of Diagnostics Spedali Civili of Brescia  Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuliana","family":"Martini","sequence":"additional","affiliation":[{"name":"Department of Diagnostics Spedali Civili of Brescia  Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Caimi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Biotechnologies Faculty of Medicine University of Brescia  Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris","family":"Ricotta","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Biotechnologies Faculty of Medicine University of Brescia  Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.395"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2010.03.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-01-405134"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02146.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/beha.2001.0133"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1182\/blood.V69.2.454.454","volume":"69","author":"Rodeghiero F.","year":"1987","journal-title":"Blood"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03661.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1182\/blood.V84.3.676.676","volume":"84","author":"Sadler J. 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