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No significant differences among single diagnoses were found both for <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>n(II) and <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>. When determined <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>n(II)\/<jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content> ratio, in controls its value was 24.6, but it was 2.6 in patients. After <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern\u2010blotting with anti\u2010<jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content> and anti\u2010<jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>n chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>\u2010 and <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>n blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimised algorithm. We constructed from those profiles decision trees that enable to distinguish different groups of tumours. The blood samples were heat\u2010treated, in which we supposed mainly <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>, but samples contained other thermostable <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>n\u2010containing proteins that were helpful for identification of embryonal tumours with 88% accuracy and for identification of sarcomas with 78% accuracy. In <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content> blots the accuracies were 53 and 45%, respectively. Simultaneous analysis of <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>n blots did not increased accuracy of identification neither in embryonal tumours (80%) nor in sarcomas. Those results are promising not only from diagnostic point of view but particularly in the area of studying of individual <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content> isoforms and their aggregates in malignant tumours.<\/jats:p>","DOI":"10.1002\/elps.201200561","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T18:58:17Z","timestamp":1361213897000},"page":"1637-1648","source":"Crossref","is-referenced-by-count":10,"title":["Use of brightness wavelet transformation for automated analysis of serum metallothioneins\u2010 and zinc\u2010containing proteins by <scp>W<\/scp>estern blots to subclassify childhood solid tumours"],"prefix":"10.1002","volume":"34","author":[{"given":"Lenka","family":"Vyslouzilova","sequence":"first","affiliation":[{"name":"Department of Cybernetics Faculty of Electrical Engineering Czech Technical University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sona","family":"Krizkova","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiri","family":"Anyz","sequence":"additional","affiliation":[{"name":"Department of Cybernetics Faculty of Electrical Engineering Czech Technical University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Hynek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Hrabeta","sequence":"additional","affiliation":[{"name":"Department of Paediatric Haematology and Oncology 2nd Medical Faculty Charles University in Prague and University Hospital Motol  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarmila","family":"Kruseova","sequence":"additional","affiliation":[{"name":"Department of Paediatric Haematology and Oncology 2nd Medical Faculty Charles University in Prague and University Hospital Motol  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Eckschlager","sequence":"additional","affiliation":[{"name":"Department of Paediatric Haematology and Oncology 2nd Medical Faculty Charles University in Prague and University Hospital Motol  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vojtech","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Stepankova","sequence":"additional","affiliation":[{"name":"Department of Cybernetics Faculty of Electrical Engineering Czech Technical University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rene","family":"Kizek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Faculty of Agronomy Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01574a064"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i7.993"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/172460080301800302"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409230091169168"},{"key":"e_1_2_7_6_1","first-page":"465","volume":"46","author":"Simpkins C. 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