{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T12:49:09Z","timestamp":1772455749727,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T00:00:00Z","timestamp":1370390400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>Methylarginines (<jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>s) are potent vasoconstrictors that have been reported to be present at elevated concentrations in the blood of patients suffering from cardiovascular disease. To determine the diagnostic potential of <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>s for cardiovascular disease, a method capable of rapidly quantifying their endogenous concentrations from serum samples is required. To that end, a heat\u2010assisted extraction method was developed. Serum was first rapidly heated, causing it to congeal into a gel, and then subjected to solid\u2013liquid extraction. The extraction solution was then derivatized with a fluorogenic dye and analyzed by <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> to permit quantitation of the <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>s. This heat\u2010assisted extraction procedure allowed a no\u2010net\u2010dilution extraction of the analytes to be performed into a solvent compatible with the subsequent <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> analysis. This enabled direct detection of low\u2010abundance analytes, such as <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>s, without the need for a preconcentration step. This sample preparation method was compared with a commonly used solid\u2010phase extraction method for <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content> analysis. Endogenous <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content> concentrations determined by both the heating and solid\u2010phase extraction methods were found to be in good agreement with each other and with values previously reported in the literature.<\/jats:p>","DOI":"10.1002\/elps.201200567","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T18:58:20Z","timestamp":1361213900000},"page":"1693-1700","source":"Crossref","is-referenced-by-count":15,"title":["Heat\u2010assisted extraction for the determination of methylarginines in serum by CE"],"prefix":"10.1002","volume":"34","author":[{"given":"Thomas H.","family":"Linz","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Kansas Lawrence KS USA"},{"name":"Ralph N. 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