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A simple, rapid, and robust procedure in terms of sample treatment, separation, and quantitation based on CE\u2010LIF has been optimized for use in human plasma samples. Time required for derivatization was 15 min and analysis time was 35 min. 4\u2010<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>luoro\u20107\u2010nitro\u20102,1,3\u2010benzoxadiazole (<jats:styled-content style=\"fixed-case\">NBD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>) was the labeling agent used for obtaining fluorescent derivatives. Electrophoretic conditions were: 175 mM borate buffer at p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> 10.25 prepared with 12.5 mM \u03b2\u2010cyclodextrin. The voltage applied was +21 kV. Fourteen amino acids could be quantified: <jats:sc>l<\/jats:sc>\u2010proline, <jats:sc>l<\/jats:sc>\u2010phenylalanine, <jats:sc>l<\/jats:sc>\u2010leucine, <jats:sc>l<\/jats:sc>\u2010isoleucine, <jats:sc>l<\/jats:sc>\u2010ornithine, <jats:sc>d<\/jats:sc>\u2010ornithine, <jats:sc>l<\/jats:sc>\u2010glutamine, <jats:sc>l<\/jats:sc>\u2010alanine, <jats:sc>l<\/jats:sc>\u2010threonine, glycine, <jats:sc>l<\/jats:sc>\u2010serine, <jats:sc>d<\/jats:sc>\u2010serine, taurine and <jats:sc>l<\/jats:sc>\u2010glutamate. With this chiral <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> method, <jats:sc>l<\/jats:sc>\u2010 and <jats:sc>d<\/jats:sc>\u2010amino acids are adequately separated. The method was validated for a representative group of amino acids in human plasma: <jats:sc>l<\/jats:sc>\u2010proline, <jats:sc>l<\/jats:sc>\u2010isoleucine, <jats:sc>l<\/jats:sc>\u2010ornithine, <jats:sc>l<\/jats:sc>\u2010glutamine, <jats:sc>l<\/jats:sc>\u2010alanine <jats:sc>l<\/jats:sc>\u2010threonine, glycine, <jats:sc>l<\/jats:sc>\u2010serine, <jats:sc>d<\/jats:sc>\u2010serine, and glutamate. The method has been successfully applied to human plasma from patients with bipolar disorder, all of whom were taking lithium as a mood stabilizer. Eleven amino acids were quantified in plasma from nine patients, aged 24\u201355 years. The results were in accordance to published values for the bipolar patients. 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