{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:01:01Z","timestamp":1791086461931,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T00:00:00Z","timestamp":1377129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>Microfluidics\u2010based nano<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> (chip<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) was used to identify and quantify proteins in epithelial lining fluid (<jats:styled-content style=\"fixed-case\">ELF<\/jats:styled-content>), collected during bronchoscopy from the main bronchi of chronic obstructive pulmonary disease (COPD) patients and healthy controls using microprobes. <jats:styled-content style=\"fixed-case\">ELF<\/jats:styled-content> is a biofluid that is well suited to study pathophysiological processes in the lung, because it contains high concentrations of biologically active molecules. 1D\u2010PAGE followed by in\u2010gel tryptic digestion and chip<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> resulted in identification of approximately 300 proteins. A comparative study of <jats:styled-content style=\"fixed-case\">ELF<\/jats:styled-content> from <jats:styled-content style=\"fixed-case\">COPD<\/jats:styled-content> patients and non\u2010<jats:styled-content style=\"fixed-case\">COPD<\/jats:styled-content> controls using chemical stable isotope labeling (i<jats:styled-content style=\"fixed-case\">TRAQ<\/jats:styled-content>\u00ae\u20108<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>lex) showed that the levels of lactotransferrin, high\u2010mobility group protein <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 (<jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content> 1), alpha 1\u2010antichymotrypsin and cofilin\u20101 differed significantly in <jats:styled-content style=\"fixed-case\">ELF<\/jats:styled-content> from <jats:styled-content style=\"fixed-case\">COPD<\/jats:styled-content> patients and non\u2010<jats:styled-content style=\"fixed-case\">COPD<\/jats:styled-content> controls (<jats:italic>p<\/jats:italic>\u2010values &lt; 0.05). These results were reproduced in another, independent set of <jats:styled-content style=\"fixed-case\">ELF<\/jats:styled-content> samples from <jats:styled-content style=\"fixed-case\">COPD<\/jats:styled-content> patients and non\u2010<jats:styled-content style=\"fixed-case\">COPD<\/jats:styled-content> controls and further validated by immunohistochemistry. This study shows the feasibility of performing chip<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> and quantitative proteomics in human <jats:styled-content style=\"fixed-case\">ELF<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/elps.201300020","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T03:05:58Z","timestamp":1369710358000},"page":"2683-2694","source":"Crossref","is-referenced-by-count":25,"title":["Proteomic analysis of human epithelial lining fluid by microfluidics\u2010based nano<scp>LC<\/scp>\u2010<scp>MS<\/scp>\/<scp>MS<\/scp>: A feasibility study"],"prefix":"10.1002","volume":"34","author":[{"given":"Lorenza","family":"Franciosi","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Analytical Biochemistry University of Groningen and Netherlands Proteomics Center  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Govorukhina","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Analytical Biochemistry University of Groningen and Netherlands Proteomics Center  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizia","family":"Fusetti","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Groningen and Netherlands Proteomics Center  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert","family":"Poolman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Groningen and Netherlands Proteomics Center  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique E.","family":"Lodewijk","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Timens","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirkje","family":"Postma","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases University Medical Center Groningen University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"ten Hacken","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases University Medical Center Groningen University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Bischoff","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Analytical Biochemistry University of Groningen and Netherlands Proteomics Center  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.06.00024505"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15412550903049140"},{"key":"e_1_2_7_4_1","first-page":"157","volume":"4","author":"Dahl M.","year":"2009","journal-title":"Int. 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