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The method involved the use of specific antibody to detect the DNA lesion (8\u2010OHdG) and consecutive fluorescence labeling. Next, urinary 8\u2010OHdG fluorescently labeled along with other constituents were resolved by capillary electrophoretic system and the lesion of interest was detected using a fluorescence detector. The limit of detection was 0.18 fmol, which proved sufficient sensitivity for detection and quantification of 8\u2010OHdG in untreated urine samples. The relative standard deviation was found to be 11.32% for migration time and 5.52% for peak area. To demonstrate the utility of this method, the urinary concentration of 8\u2010OHdG in an Alzheimer's transgenic mouse model was determined. Collectively, our results indicate that this methodology offers great advantages, such as high separation efficiency, good selectivity, low limit of detection, simplicity and low cost of analysis.<\/jats:p>","DOI":"10.1002\/elps.201300036","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T03:09:02Z","timestamp":1369710542000},"page":"2268-2274","source":"Crossref","is-referenced-by-count":42,"title":["Detection and quantification of 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine in Alzheimer's transgenic mouse urine using capillary electrophoresis"],"prefix":"10.1002","volume":"34","author":[{"given":"Cheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurosciences UCSD School of Medicine La Jolla CA USA"},{"name":"Department of Biomedical Engineering Louisiana Tech University Ruston LA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gergana","family":"Nestorova","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Louisiana Tech University Ruston LA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Rissman","sequence":"additional","affiliation":[{"name":"Department of Neurosciences UCSD School of Medicine La Jolla CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"June","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Louisiana Tech University Ruston LA USA"},{"name":"Division of Technology Engineering and Mathematics Bossier Parish Community College Bossier City LA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,15]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"97","volume":"3","author":"Mohammad Arif Y. 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