{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T09:10:52Z","timestamp":1767863452065,"version":"3.49.0"},"reference-count":65,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,2]],"date-time":"2013-12-02T00:00:00Z","timestamp":1385942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is a protozoan parasite infecting almost all warm\u2010blooded animals and humans. There are three infective stages of <jats:italic>T. gondii<\/jats:italic>: the tachyzoites, the bradyzoites, and the oocysts. The tachyzoite is a rapidly multiplying stage and the main pathogenic factor. In <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>orth <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>merica and <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>urope, <jats:italic>T. gondii<\/jats:italic> is consisted of four major clonal lineages (namely Types I, II, III, and Type 12). In this study, we explored the proteomic profiles of different genotypes (Type I\u2010<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> strain, Type II\u2010<jats:styled-content style=\"fixed-case\">PRU<\/jats:styled-content> strain, Type II\u2010<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">QHO<\/jats:styled-content> strain, and <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>oxo<jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content> 9\u2010TgC7 strain) of <jats:italic>T. gondii<\/jats:italic> tachyzoites by using 2D DIGE combined with <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content>. Totally, 110 differentially abundant protein spots were selected. Of these, 98 spots corresponding to 56 proteins from <jats:italic>T. gondii<\/jats:italic> were successfully identified. These included surface antigen (<jats:styled-content style=\"fixed-case\">SAG<\/jats:styled-content>1), heat shock protein 70 (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>sp 70), disulfide isomerase, coronin, heat shock protein 60 (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>sp 60), pyruvate kinase, receptor for activated <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> kinase 1, and peroxiredoxin. Gene ontology enrichment analysis revealed that most of the differentially abundant proteins were involved in biological regulation, metabolic process, response to stress, binding, antioxidant activity, and transporter activity. According to the KEGG metabolic pathway maps of <jats:italic>T. gondii<\/jats:italic>, some identified proteins were involved in the glycolytic\/gluconeogenesis pathway. The present study identified differentially abundant proteins among different genotypes of <jats:italic>T. gondii<\/jats:italic> and these findings have implications for the better understanding of the phenotypic differences among the examined <jats:italic>T. gondii<\/jats:italic> genotypes, which in turn may contribute to the better control of toxoplasmosis.<\/jats:p>","DOI":"10.1002\/elps.201300044","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T08:45:02Z","timestamp":1382604302000},"page":"533-545","source":"Crossref","is-referenced-by-count":20,"title":["Comparative proteomic analysis of different <i><scp>T<\/scp>oxoplasma gondii<\/i> genotypes by two\u2010dimensional fluorescence difference gel electrophoresis combined with mass spectrometry"],"prefix":"10.1002","volume":"35","author":[{"given":"Dong\u2010Hui","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences  Lanzhou Gansu Province P. 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