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It attains an ester linkage that can be hydrolyzed in alkali conditions. The separation performance of the new gel system was tested by 1D and 2D SDS\u2010PAGE using the outer chloroplast envelope of <jats:italic>Pisum sativum<\/jats:italic> as well as a soluble protein fraction of human lymphocytes, respectively. Gel spot staining (CBB), dissolving, and extracting were conducted using a custom\u2010developed workflow. It includes protein extraction with an ammonia\u2013SDS buffer followed by methanol treatment to remove acrylamide filaments. Necessary purification for MALDI\u2010TOF analysis was implemented using methanol\u2013chloroform precipitation and perfusion HPLC. Both cleaning procedures were applied to several standard proteins of different molecular weight as well as \u2018real\u2019 biological samples (8\u201375 kDa). 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