{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:04:52Z","timestamp":1761581092892,"version":"build-2065373602"},"reference-count":61,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2013,8,2]],"date-time":"2013-08-02T00:00:00Z","timestamp":1375401600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>In the present study, we undertook a molecular epidemiological survey of <jats:italic>Cryptosporidium<\/jats:italic> and <jats:italic>Giardia<\/jats:italic> in calves on three dairy and two beef farms within an open drinking water catchment area (Melbourne, Australia). Faecal samples (<jats:italic>n<\/jats:italic> = 474) were collected from calves at two time points (5 months apart) and tested using a PCR\u2010based mutation scanning\u2010targeted sequencing phylogenetic approach, employing regions within the genes of small subunit (SSU) of ribosomal RNA (designated partial SSU), 60 kDa glycoprotein (p<jats:italic>gp60<\/jats:italic>) and triose phosphate isomerase (p<jats:italic>tpi<\/jats:italic>) as genetic markers. Using partial SSU, the <jats:italic>C. bovis<\/jats:italic>, <jats:italic>C. parvum<\/jats:italic>, <jats:italic>C. ryanae<\/jats:italic> and a new genotype of <jats:italic>Cryptosporidium<\/jats:italic> were characterised from totals of 74 (15.6%), 35 (7.3%), 37 (7.8%) and 9 (1.9%) samples, respectively. Using p<jats:italic>gp60<\/jats:italic>, <jats:italic>C. parvum<\/jats:italic> genotype IIa subgenotype A18G3R1 was detected in 29 samples. Using p<jats:italic>tpi<\/jats:italic>, <jats:italic>G. duodenalis<\/jats:italic> assemblages A and E were detected in totals of 10 (2.1%) and 130 (27.4%) samples, respectively. The present study showed that a considerable proportion of dairy and beef calves in this open water catchment region excreted <jats:italic>Cryptosporidium<\/jats:italic> (i.e. subgenotype IIaA18G3R1) and <jats:italic>Giardia<\/jats:italic> (e.g. assemblage A) that are consistent with those infecting humans, inferring that they are of zoonotic importance. Future work should focus on exploring, in a temporal and spatial way, whether these parasites occur in the environment and water of the catchment reservoir.<\/jats:p>","DOI":"10.1002\/elps.201300146","type":"journal-article","created":{"date-parts":[[2013,5,26]],"date-time":"2013-05-26T07:06:47Z","timestamp":1369552007000},"page":"2259-2267","source":"Crossref","is-referenced-by-count":25,"title":["Assessing calves as carriers of <i>Cryptosporidium<\/i> and <i>Giardia<\/i> with zoonotic potential on dairy and beef farms within a water catchment area by mutation scanning"],"prefix":"10.1002","volume":"34","author":[{"given":"Harshanie","family":"Abeywardena","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron R.","family":"Jex","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon M.","family":"Firestone","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"McPhee","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Driessen","sequence":"additional","affiliation":[{"name":"Melbourne Water Corporation  Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anson V.","family":"Koehler","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shane R.","family":"Haydon","sequence":"additional","affiliation":[{"name":"Melbourne Water Corporation  Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"von Samson\u2010Himmelstjerna","sequence":"additional","affiliation":[{"name":"Institute of Parasitology and Tropical Veterinary Medicine  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melita A.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Melbourne Water Corporation  Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.09.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(04)58002-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.09.022"},{"key":"e_1_2_7_5_1","first-page":"536","volume-title":"Oxford Textbook of Zoonoses","author":"Jex A. 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