{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:57:19Z","timestamp":1760576239536,"version":"build-2065373602"},"reference-count":13,"publisher":"Wiley","issue":"20-21","license":[{"start":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T00:00:00Z","timestamp":1379548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>Long\u2010term stored <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> can be sometimes the only source of genetic material of an organism that does not exist anymore, but a research interest still persists. However, there is a lack of information about useful methods to improve quality from such type of material. In this study, we compared four different protocols using <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples collected in 1998. Fresh <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was also tested aiming to check the differences between these two material types. Sixteen samples of each <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> type treated with phenol\u2010chloroform with <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content> 5.0%, silica\u2010gel membrane spin column, <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content> 7.5%, and glass\u2010fiber matrix spin column were submitted to spectrophotometer measurements, electrophoresis, <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> to assess the best method concerning yield, quality, and purity. Based on the results, purification with <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content> 7.5% was considered the best method to treat aged <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples. In addition to the efficiency, this protocol has low cost. Analyzing the data, we also conclude that long\u2010term stored <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> may be considered a reliable and potential resource for future molecular studies.<\/jats:p>","DOI":"10.1002\/elps.201300245","type":"journal-article","created":{"date-parts":[[2013,7,26]],"date-time":"2013-07-26T08:13:38Z","timestamp":1374826418000},"page":"3039-3045","source":"Crossref","is-referenced-by-count":1,"title":["Treatment of long\u2010term stored DNA\u2014Comparison between different methods to obtain high\u2010quality material"],"prefix":"10.1002","volume":"34","author":[{"given":"M\u00e1ira Pedroso","family":"de Almeida","sequence":"first","affiliation":[{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Souza","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iuri Viotti","family":"P\u00e9riss\u00e9","sequence":"additional","affiliation":[{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio","family":"de Souza Duarte","sequence":"additional","affiliation":[{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Veroneze","sequence":"additional","affiliation":[{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone E.","family":"Facioni Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2010.10.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1060"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac000225s"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35072071"},{"key":"e_1_2_6_6_1","first-page":"T01392","author":"Gaillard C.","year":"1998","journal-title":"Tech. Tips Online"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.03.014"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.6.1346"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/2.3.383"},{"volume-title":"Molecular Cloning: A Laboratory Manual","year":"1989","author":"Sambrook J.","key":"e_1_2_6_10_1"},{"key":"e_1_2_6_11_1","first-page":"25","volume":"15","author":"Pires A. V.","year":"2006","journal-title":"Arch. Latinoam. Prod. Anim."},{"volume-title":"Molecular Cloning: A Laboratory Manual","year":"2001","author":"Sambrook J.","key":"e_1_2_6_12_1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0388.2006.00611.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782009000500044"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201300245","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201300245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:25:41Z","timestamp":1760549141000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201300245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,19]]},"references-count":13,"journal-issue":{"issue":"20-21","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1002\/elps.201300245"],"URL":"https:\/\/doi.org\/10.1002\/elps.201300245","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2013,9,19]]}}}