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In this study, a sensitive and selective method for free <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> quantification in cerebrospinal fluid (<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>) has been standardised. This method is based on <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content> detection using 4\u2010fluoro\u20107\u2010nitrobenzo\u20102\u2010oxa\u20101,3\u2010diazole (NBD\u2010F) as a derivatisating agent. The reaction conditions (NBD\u2010F concentration, pH, temperature and reaction time) and the electrophoretic parameters (run buffer composition and pH and separation voltage) were optimised to obtain the maximum derivatisation efficiency and electrophoretic resolution. The best resolution was obtained using 200 mM sodium borate, 10 mM SDS, 8.5 mM \u03b2\u2010CD, pH 10 and 20 kV voltage. The method was linear in the concentration range of 2.5\u20131000 nM with good inter\u2010 and intra\u2010assay precision values. The effects of <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> handling on free <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> concentrations were also evaluated. Our results show that the time delay between <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> collection and freezing strongly increases the <jats:styled-content style=\"fixed-case\">CSF GABA<\/jats:styled-content> values. Age\u2010related reference values were established in 55 paediatric controls. The influence of antiepileptic therapy on free <jats:styled-content style=\"fixed-case\">CSF GABA<\/jats:styled-content> was studied in 38 neuropaediatric patients. Significantly, higher <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> values were obtained in patients taking valproic acid or vigabatrin therapy, which are antiepileptic drugs that modulate <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> metabolism.<\/jats:p>","DOI":"10.1002\/elps.201300261","type":"journal-article","created":{"date-parts":[[2013,12,14]],"date-time":"2013-12-14T21:22:40Z","timestamp":1387056160000},"page":"1181-1187","source":"Crossref","is-referenced-by-count":12,"title":["Analysis of cerebrospinal fluid \u03b3\u2010aminobutyric acid by capillary electrophoresis with laser\u2010induced fluorescence detection"],"prefix":"10.1002","volume":"35","author":[{"given":"Mercedes","family":"Casado","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry and Neuropaediatrics Hospital Sant Joan de D\u00e9u\u2010CIBERER\u2010ISCIII  Barcelona Spain"},{"name":"Department of Biochemistry and Molecular Biology UAB  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Molero","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Neuropaediatrics Hospital Sant Joan de D\u00e9u\u2010CIBERER\u2010ISCIII  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Sierra","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Neuropaediatrics Hospital Sant Joan de D\u00e9u\u2010CIBERER\u2010ISCIII  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angels","family":"Garc\u00eda\u2010Cazorla","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Neuropaediatrics Hospital Sant Joan de D\u00e9u\u2010CIBERER\u2010ISCIII  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aida","family":"Ormazabal","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Neuropaediatrics Hospital Sant Joan de D\u00e9u\u2010CIBERER\u2010ISCIII  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Artuch","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Neuropaediatrics Hospital Sant Joan de D\u00e9u\u2010CIBERER\u2010ISCIII  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:NERE.0000018836.82122.23"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026115607216"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-04-01548.2003"},{"key":"e_1_2_8_5_1","first-page":"PR1","volume":"8","author":"Dhossche D.","year":"2002","journal-title":"Med. 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