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The proteins were analyzed at different time points after infection with 2DE followed by MALDI\u2010TOF\/TOF identification. Comparative analysis of multiple 2DE gels revealed that the majority of changes in protein abundance appeared between 48 and 96 h after infection. MS identified 44 altered proteins, including 20 proteins increased in abundance and 24 proteins decreased in abundance, which were found to be involved in cytoskeleton, signal transduction, energy metabolism, host macromolecular biosynthesis, and stress response. Moreover, 22 genes corresponding to the altered proteins were quantified by real\u2010time RT\u2010PCR to examine the transcriptional profiles between infected and uninfected THP\u20101\u2010derived macrophages. Finally, we mapped the altered pathways and networks using ingenuity pathway analysis, which suggested that the altered protein species were heavily favored germ cell\u2010Sertoli cell junction signaling as the primary pathway. Furthermore, mechanisms of viral exit from host cell and macrophage stimulating protein\u2010recepteur d'origine nantais signaling appeared to be major pathways modulated in infected cells. This study effectively provides useful dynamic protein\u2010related information concerning <jats:italic>B. abortus<\/jats:italic> infection in macrophages.<\/jats:p>","DOI":"10.1002\/elps.201300378","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T07:21:28Z","timestamp":1392189688000},"page":"1130-1143","source":"Crossref","is-referenced-by-count":6,"title":["Comparative proteomics analysis of host cells infected with <i>Brucella abortus<\/i><scp>A<\/scp>19"],"prefix":"10.1002","volume":"35","author":[{"given":"Yongping","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Sciences and Technology Zhejiang A&amp;F University  Hangzhou P.R. 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