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A DEP approach utilizing a microarray device now allows rapid isolation of ccf\u2010DNA directly from a small volume of unprocessed blood. In this study, the DEP device is used to compare the ccf\u2010DNA isolated directly from whole blood and plasma from 11 chronic lymphocytic leukemia (CLL) patients and one normal individual. Ccf\u2010DNA from both blood and plasma samples was separated into DEP high\u2010field regions, after which cells (blood), proteins, and other biomolecules were removed by a fluidic wash. The concentrated ccf\u2010DNA was detected on\u2010chip by fluorescence, and then eluted for PCR and DNA sequencing. The complete process from blood to PCR required less than 10 min; an additional 15\u00a0min was required to obtain plasma from whole blood. Ccf\u2010DNA from the equivalent of 5\u00a0\u03bcL of CLL blood and 5\u00a0\u03bcL of plasma was amplified by PCR using Ig heavy\u2010chain variable (IGHV) specific primers to identify the unique IGHV gene expressed by the leukemic B\u2010cell clone. The PCR and DNA sequencing results obtained by DEP from all 11 CLL blood samples and from 8 of the 11 CLL plasma samples were exactly comparable to the DNA sequencing results obtained from genomic DNA isolated from CLL patient leukemic B cells (gold standard).<\/jats:p>","DOI":"10.1002\/elps.201400016","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T12:01:22Z","timestamp":1397131282000},"page":"1828-1836","source":"Crossref","is-referenced-by-count":58,"title":["Dielectrophoretic isolation and detection of cancer\u2010related circulating cell\u2010free DNA biomarkers from blood and plasma"],"prefix":"10.1002","volume":"35","author":[{"given":"Avery","family":"Sonnenberg","sequence":"first","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer Y.","family":"Marciniak","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine A.","family":"Skowronski","sequence":"additional","affiliation":[{"name":"Department of Nanoengineering University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sareh","family":"Manouchehri","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rassenti","sequence":"additional","affiliation":[{"name":"UCSD Moores Cancer Center University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela M.","family":"Ghia","sequence":"additional","affiliation":[{"name":"UCSD Moores Cancer Center University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"George F.","family":"Widhopf","sequence":"additional","affiliation":[{"name":"UCSD Moores Cancer Center University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Kipps","sequence":"additional","affiliation":[{"name":"UCSD Moores Cancer Center University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Heller","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla CA USA"},{"name":"Department of Nanoengineering University of California San Diego  La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3066"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2006.10.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-7372(02)00077-4"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.02.006"},{"key":"e_1_2_8_6_1","first-page":"307","volume":"2","author":"Board R. 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