{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:57:32Z","timestamp":1760576252232,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"21-22","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["2011-0030049"],"award-info":[{"award-number":["2011-0030049"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:p>DNA degradation is a major obstacle in gaining an accurate profile with standard DNA typing technology. Although alternative genotyping strategies such as mini\u2010STRs and SNPs have proven to be more successful in profiling degraded DNA, these approaches also have limitations. Here, we show that locus enrichment by hybridization of degraded genomic DNA with an STR locus\u2010specific biotinylated oligonucleotide is a powerful approach to overcome problems in STR typing of highly degraded DNA. An experimental investigation of factors affecting the efficiency of this method indicates that the choice of primer and molar ratio of primers to genomic DNA are critical factors in improving enrichment of the STR locus before genotyping with multiplex kits. In addition, we find that indirect capture rather than direct capture with magnetic beads yields better enrichment efficiency for STR locus enrichments. Using these strategies, we demonstrate an improvement in STR typing of DNA from cultured cells damaged by exposure to sunlight or UV. We suggest that this approach could be applied to highly degraded forensic samples alone or in combination with mini\u2010STRs.<\/jats:p>","DOI":"10.1002\/elps.201400102","type":"journal-article","created":{"date-parts":[[2014,8,20]],"date-time":"2014-08-20T21:23:12Z","timestamp":1408569792000},"page":"3158-3164","source":"Crossref","is-referenced-by-count":2,"title":["Optimization of STR locus enrichment for STR profiling of fragmented DNA"],"prefix":"10.1002","volume":"35","author":[{"given":"Seon\u2010Kyu","family":"Ham","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Sungkyunkwan University  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Se\u2010Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"DNA Analysis Laboratory Division of Forensic DNA Supreme Prosecutors\u2019 Office  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jang\u2010Won","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Sungkyunkwan University  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo Young","family":"Seo","sequence":"additional","affiliation":[{"name":"DNA Analysis Laboratory Division of Forensic DNA Supreme Prosecutors\u2019 Office  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang\u2010Man","family":"Woo","sequence":"additional","affiliation":[{"name":"DNA Analysis Laboratory Division of Forensic DNA Supreme Prosecutors\u2019 Office  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheol Yong","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Sungkyunkwan University  Suwon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Hwan","family":"Lee","sequence":"additional","affiliation":[{"name":"DNA Analysis Laboratory Division of Forensic DNA Supreme Prosecutors\u2019 Office  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112582"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-461-2_1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13353-011-0068-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2008.04.004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000334982"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0379-0738(88)90118-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2009.09.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-004-2681-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(97)00129-1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2003269"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1556-4029.2007.00584.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2003043"},{"key":"e_1_2_7_14_1","first-page":"540","volume":"46","author":"Alonso A.","year":"2005","journal-title":"Croat. 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