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In this work, a metabolomic approach based on ultrafiltration and analysis by CE\u2010MS has been used to obtain representative fingerprints of polar metabolites from serum samples in order to distinguish between patients with Alzheimer's disease, mild cognitive impairment, and healthy controls. By the use of partial least squares discriminant analysis it was possible to classify patients according to the disease stage and then identify potential markers. Significant increase was observed with progression of disease in levels of choline, creatinine, asymmetric dimethyl\u2010arginine, homocysteine\u2010cysteine disulfide, phenylalanyl\u2010phenylalanine, and different medium chain acylcarnitines. On the other hand, asparagine, methionine, histidine, carnitine, acetyl\u2010spermidine, and C5\u2010carnitine were reduced in these serum samples. In this way, multiple essential pathways were found implicated in the underlying pathology, such as oxidative stress or defects in energy metabolism. However, the most interesting results are related to the association of several vascular risk factors with Alzheimer's disease.<\/jats:p>","DOI":"10.1002\/elps.201400196","type":"journal-article","created":{"date-parts":[[2014,8,18]],"date-time":"2014-08-18T20:59:44Z","timestamp":1408395584000},"page":"3321-3330","source":"Crossref","is-referenced-by-count":113,"title":["Metabolomic profiling of serum in the progression of Alzheimer's disease by capillary electrophoresis\u2013mass spectrometry"],"prefix":"10.1002","volume":"35","author":[{"given":"Ra\u00fal","family":"Gonz\u00e1lez\u2010Dom\u00ednguez","sequence":"first","affiliation":[{"name":"Department of Chemistry and CC.MM Faculty of Experimental Science University of Huelva  Huelva Spain"},{"name":"Campus of Excellence International ceiA3 University of Huelva  Spain"},{"name":"Research Center of Health and Environment (CYSMA) University of Huelva  Huelva Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonia","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Center for Metabolomics and Bioanalysis (CEMBIO), Pharmacy Faculty Universidad San Pablo CEU  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara","family":"Garc\u00eda\u2010Barrera","sequence":"additional","affiliation":[{"name":"Department of Chemistry and CC.MM Faculty of Experimental Science University of Huelva  Huelva Spain"},{"name":"Campus of Excellence International ceiA3 University of Huelva  Spain"},{"name":"Research Center of Health and Environment (CYSMA) University of Huelva  Huelva Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coral","family":"Barbas","sequence":"additional","affiliation":[{"name":"Center for Metabolomics and Bioanalysis (CEMBIO), Pharmacy Faculty Universidad San Pablo CEU  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"G\u00f3mez\u2010Ariza","sequence":"additional","affiliation":[{"name":"Department of Chemistry and CC.MM Faculty of Experimental Science University of Huelva  Huelva Spain"},{"name":"Campus of Excellence International ceiA3 University of Huelva  Spain"},{"name":"Research Center of Health and Environment (CYSMA) University of Huelva  Huelva Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1212\/WNL.34.7.939","volume":"34","author":"McKahnn G.","year":"1984","journal-title":"Neurology"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70178-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.7.5.635"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.58.12.1985"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(03)00262-X"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2788.2005.00704.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2011.11.075"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.52.8.1555"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.15.1777"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(00)00124-X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.4.2.189"},{"key":"e_1_2_7_13_1","volume-title":"Metabolic profiling: Its Role in Biomarker Discovery and Gene Function Analysis","author":"Beecher C. 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