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In the present study, we genetically characterized 82 samples of <jats:italic>Cryptosporidium<\/jats:italic> from humans following conventional coproscopic testing in a routine, diagnostic laboratory. Using a PCR\u2010coupled single\u2010strand conformation polymorphism (SSCP) technique, targeting portions of the small subunit rRNA (<jats:italic>SSU<\/jats:italic>), and 60\u00a0kDa glycoprotein (<jats:italic>gp60<\/jats:italic>) loci, we identified two species of <jats:italic>Cryptosporidium<\/jats:italic>, including <jats:italic>C. hominis<\/jats:italic> (subgenotypes IbA10G2, IdA16, IeA12G3T3, and IfA19G1) and <jats:italic>C. parvum<\/jats:italic> (IIaA16G1R1 and IIaA18G3), and a new operational taxonomic unit (OTU) that genetically closely resembled <jats:italic>C. wrairi<\/jats:italic>. This OTU was further characterized using markers in the <jats:italic>actin<\/jats:italic>, <jats:italic>Cryptosporidium<\/jats:italic> oocyst wall protein (<jats:italic>COWP<\/jats:italic>), and 70 kDa heat shock protein (<jats:italic>hsp70<\/jats:italic>) genes. This study provides the first characterization of species and genotypes of <jats:italic>Cryptosporidium<\/jats:italic> from Tasmania, and presents clear genetic evidence, using five independent genetic loci, for a new genotype or species of <jats:italic>Cryptosporidium<\/jats:italic> in a Tasmanian person with a recent history of travelling to Bali, Indonesia. It would be interesting to undertake detailed molecular\u2010based studies of <jats:italic>Cryptosporidium<\/jats:italic> in Indonesia and neighbouring countries, in conjunction with morphological and experimental investigations of new genotypes.<\/jats:p>","DOI":"10.1002\/elps.201400225","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T23:13:07Z","timestamp":1402441987000},"page":"2600-2607","source":"Crossref","is-referenced-by-count":32,"title":["First genetic analysis of <i>Cryptosporidium<\/i> from humans from Tasmania, and identification of a new genotype from a traveller to Bali"],"prefix":"10.1002","volume":"35","author":[{"given":"Anson V.","family":"Koehler","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science University of Melbourne  Werribee Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Whipp","sequence":"additional","affiliation":[{"name":"Microbiological Diagnostic Unit Public Health Laboratory, Peter Doherty Institute University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoff","family":"Hogg","sequence":"additional","affiliation":[{"name":"Microbiological Diagnostic Unit Public Health Laboratory, Peter Doherty Institute University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane R.","family":"Haydon","sequence":"additional","affiliation":[{"name":"Melbourne Water Corporation  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melita A.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Melbourne Water Corporation  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron R.","family":"Jex","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science University of Melbourne  Werribee Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science University of Melbourne  Werribee Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.07.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-1562-6_1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00076-12"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/591539"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.01.002"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.13.119990"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.02.003"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.04.028"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1505.081419"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.04.016"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1411.080239"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.01.011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00424-13"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00615-11"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00616-11"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01341-08"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.02.006"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.01.010"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.02.012"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00116-08"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201300100"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.02.017"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600772"},{"key":"e_1_2_8_25_1","first-page":"76","volume":"16","author":"Hawkesford T.","year":"1995","journal-title":"Aust. 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