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The study comprised 30 patients with PBC; 20 AMA\u2010positive and ten AMA\u2010negative patients matched for age, sex<jats:bold>,<\/jats:bold> and pathological stage. A screening group (four AMA\u2010positive and four AMA\u2010negative patients) was used for 2D\u2010DIGE. Protein spots that were differently abundant between the two groups were identified via dye intensity and MS. Nine candidate proteins were identified from these spots. Western blotting was used to verify two of the identified proteins, serum amyloid P\u2010component (SAP) and vitronectin (VN). VN levels were significantly higher in the sera of AMA\u2010negative PBC patients (<jats:italic>p<\/jats:italic> &lt;\u00a00.01), whereas no significant difference was found between the two groups for SAP. To our knowledge, this is the first study to use serological comparative proteomics to explore differences between AMA\u2010positive and \u2010negative PBC patients. VN levels were higher in AMA\u2010negative PBC patients, and this finding could be related to the more severe bile duct destruction observed in this group.<\/jats:p>","DOI":"10.1002\/elps.201400342","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T06:53:20Z","timestamp":1429080800000},"page":"1588-1595","source":"Crossref","is-referenced-by-count":6,"title":["Serological comparative proteomics analysis of mitochondrial autoantibody\u2010negative and \u2010positive primary biliary cirrhosis"],"prefix":"10.1002","volume":"36","author":[{"given":"Chuiwen","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaojun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shulan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education  Beijing P. R. 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