{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:46:20Z","timestamp":1760575580169,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T00:00:00Z","timestamp":1426118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003549","name":"Orsz\u00e1gos Tudom\u00e1nyos Kutat\u00e1si Alapprogramok","doi-asserted-by":"publisher","award":["K83766"],"award-info":[{"award-number":["K83766"]}],"id":[{"id":"10.13039\/501100003549","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MTA-PE Translational Glycomics Grant","award":["97101"],"award-info":[{"award-number":["97101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Despite of the fact that the Human Genome Project was completed more than a decade ago, identification of the genetic background of polygenic diseases is still challenging. Several somewhat different approaches are available to investigate inheritable factors of complex phenotypes, all require, however efficient, high\u2010throughput techniques for SNP genotyping. In this paper, we report a robust and reliable multiplex PCR\u2013RFLP for genotype and haplotype analysis of six SNPs (rs41270082, rs3748051, rs142027015, rs3748048, rs73404011, and rs72925892) of the colipase (<jats:italic>CLPS<\/jats:italic>) gene. A multicapillary (12 capillaries) electrophoresis unit was used for high throughput and sensitive analysis of the digestion fragments. A Microsoft Excel\u2010based spreadsheet was designed for the flexible visualization and evaluation of the electrophoretic separations, which is readily adaptable for any kind of electrophoresis application. Haplotype analysis of the two loci localized in close proximity of each other was carried out by molecular method, extended haplotypes including all five SNPs in the 5\u2032 upstream region were calculated. The techniques were applied in a case\u2013control association study of type 2 diabetes mellitus. Although, single marker analysis did not reveal any significant association, it was observed that the rare GGCCG haplotype of the five 5\u2032 upstream region SNPs was about three times more frequent among patients compared to healthy control population. Our results demonstrated the applicability of multicapillary CGE in large\u2010scale, high\u2010throughput SNP analysis, and suggested that the <jats:italic>CLPS<\/jats:italic> gene polymorphisms might be considered as genetic risk factor for type 2 diabetes mellitus.<\/jats:p>","DOI":"10.1002\/elps.201400551","type":"journal-article","created":{"date-parts":[[2014,12,11]],"date-time":"2014-12-11T09:20:36Z","timestamp":1418289636000},"page":"1237-1243","source":"Crossref","is-referenced-by-count":1,"title":["Rapid analysis of colipase gene variants by multicapillary electrophoresis"],"prefix":"10.1002","volume":"36","author":[{"given":"Zsuzsanna","family":"Jacz\u00f3","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry Molecular Biology and Pathobiochemistry Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter","family":"P\u00e1l","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry Molecular Biology and Pathobiochemistry Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9ka","family":"D\u00e9nes","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry Molecular Biology and Pathobiochemistry Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anik\u00f3","family":"Somogyi","sequence":"additional","affiliation":[{"name":"2<sup>nd<\/sup> Department of Internal Medicine Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1ria","family":"Sasv\u00e1ri\u2010Sz\u00e9kely","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry Molecular Biology and Pathobiochemistry Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e1s","family":"Guttman","sequence":"additional","affiliation":[{"name":"Horv\u00e1th Laboratory of Bioseparation Sciences University of Debrecen  Debrecen Hungary"},{"name":"MTA\u2010PE Translational Glycomics Group University of Pannonia  Veszpr\u00e9m Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsolt","family":"R\u00f3nai","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry Molecular Biology and Pathobiochemistry Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058040"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/456018a"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051408"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2013.161"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.04.031"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-6-54"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201200073"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cc40680f"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/s120608100"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600568"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.02.062"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201000536"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.071159"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800755"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201300221"},{"volume-title":"International Diabetes Federation. 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