{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:53:40Z","timestamp":1789880020206,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2015,8,21]],"date-time":"2015-08-21T00:00:00Z","timestamp":1440115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>One of the main limitations of untargeted metabolomics analysis is the low detection coverage of analytical techniques such as NMR, LC\u2013MS, or GC\u2013MS. In this research, the detection coverage of an automated approach configured by the on\u2010line coupling of SPE to LC\u2013MS\/MS was evaluated by combination of sorbents based on different retention mechanisms. The approach was applied to the analysis of human serum using three types of sorbents: alkyl bonded silica, polymeric resins, and mixed\u2010mode ionic resins. The combination of four sorbents (C18, a modified polystyrene\u2013divinylbenzene resin and two mixed\u2010mode ionic resins) led to the best extraction results and, therefore, the best detection coverage, which is explained by their complementary retention mechanisms. However, some of the sorbents provide a high detection coverage by themselves, as is the case with C18, which can afford to retain almost 83% of all detected entities. Taking into account the complementarity between pairs of these sorbents (C18 and the polystyrene\u2013divinylbenzene resin with the mixed\u2010mode ionic resins), dual cartridge SPE\u2013LC\u2013MS\/MS configurations were designed for serum analysis. These configurations allowed increasing the detection coverage up to 91% of the total number of molecular features detected with all sorbents tested. An additional benefit of the SPE\u2013LC\u2013MS\/MS strategy was the improvement of sensitivity as compared to protein precipitation and fractionation with methanol and chloroform. Thus, an average preconcentration factor of 10\u201375 was obtained in the SPE\u2010based approach versus the two\u2010phase protocol for metabolites extraction.<\/jats:p>","DOI":"10.1002\/elps.201500060","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T06:06:33Z","timestamp":1434693993000},"page":"2179-2187","source":"Crossref","is-referenced-by-count":13,"title":["Enhancing detection coverage in untargeted metabolomics analysis by solid\u2010phase extraction on\u2010line coupled to LC\u2013MS\/MS"],"prefix":"10.1002","volume":"36","author":[{"given":"M\u00f3nica","family":"Calder\u00f3n\u2010Santiago","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales University of C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Maim\u00f3nides Institute of Biomedical Research (IMIBIC), Reina Sof\u00eda University Hospital University of C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feliciano","family":"Priego\u2010Capote","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales University of C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Maim\u00f3nides Institute of Biomedical Research (IMIBIC), Reina Sof\u00eda University Hospital University of C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda D. 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