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Here, we analyzed for the first time by MS the advantages of using the OFFGEL technique in the separation of the venom components of the Egyptian Elapidae <jats:italic>Walterinnesia aegyptia<\/jats:italic> snake compared to two classical methods of separation, SEC and RP\u2010HPLC. We demonstrate that OFFGEL separates venom components over a larger scale of fractions, preserve respectable resolution with regard to the presence of a given compound in adjacent fractions and allows the identification of a greater number of ions by MS (102 over 134 total ions). We also conclude that applying several separating techniques (SEC and RP\u2010HPLC in addition to OFFGEL) provides complementary results in terms of ion detection (21 more for SEC and 22 more with RP\u2010HPLC). As a result, we provide a complete list of 134 ions present in the venom of <jats:italic>W. aegyptia<\/jats:italic> by using all these techniques combined.<\/jats:p>","DOI":"10.1002\/elps.201500207","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T16:30:46Z","timestamp":1436891446000},"page":"2594-2605","source":"Crossref","is-referenced-by-count":14,"title":["Fractionation and proteomic analysis of the <i>Walterinnesia aegyptia<\/i> snake venom using OFFGEL and MALDI\u2010TOF\u2010MS techniques"],"prefix":"10.1002","volume":"36","author":[{"given":"Tarek Mohamed Abd El","family":"Aziz","sequence":"first","affiliation":[{"name":"INSERM U836 Grenoble Neuroscience Institute, LabEx Ion Channels, Science and Therapeutics  France"},{"name":"University Grenoble Alpes  Grenoble France"},{"name":"Zoology Department, Faculty of Science Minia University  El\u2010Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Bourgoin\u2010Voillard","sequence":"additional","affiliation":[{"name":"University Grenoble Alpes  Grenoble France"},{"name":"INSERM, IAB, PROMETHEE Proteomic Platform  Grenoble France"},{"name":"CHU de Grenoble IAB, Institut de Biologie et de Pathologie, PROMETHEE Proteomic Platform  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Combemale","sequence":"additional","affiliation":[{"name":"Smartox Biotechnology  Saint\u2010Martin d'H\u00e8res France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9my","family":"Beroud","sequence":"additional","affiliation":[{"name":"Smartox Biotechnology  Saint\u2010Martin d'H\u00e8res France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Fadl","sequence":"additional","affiliation":[{"name":"Zoology Department, Faculty of Science Minia University  El\u2010Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Seve","sequence":"additional","affiliation":[{"name":"University Grenoble Alpes  Grenoble France"},{"name":"INSERM, IAB, PROMETHEE Proteomic Platform  Grenoble France"},{"name":"CHU de Grenoble IAB, Institut de Biologie et de Pathologie, PROMETHEE Proteomic Platform  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"De Waard","sequence":"additional","affiliation":[{"name":"INSERM U836 Grenoble Neuroscience Institute, LabEx Ion Channels, Science and Therapeutics  France"},{"name":"University Grenoble Alpes  Grenoble France"},{"name":"Smartox Biotechnology  Saint\u2010Martin d'H\u00e8res France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66913-2_9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(99)00127-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(94)00115-E"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.08.040168.001503"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2614"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.12.010"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00097-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2013.03.020"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01514"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2009.01.001"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.02.030"},{"key":"e_1_2_7_13_1","first-page":"1031","volume":"31","author":"Hung C. 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